Table 1. Strains and plasmids

Strain or plasmid Relevant characteristics Source or reference

M. loti
MAFF303099 Wild-type Genetic Resource Center, National Institute of Agrobiological Sciences, Tsukuba, Japan
ML001 MAFF303099 Smr H. Mitsui
MYa179 ML001 cep : : TnphoA This study
YML1011 ML001 cep : : aadA This study
YML1012 ML001 {Delta}ndvB : : aadA This study
YML1006 ML001 {Delta}ndvA : : aacC1 This study
SRM-9 Symbiotic pseudorevertant of MYa179 This study
SRM-10 Symbiotic pseudorevertant of MYa179 This study
SRM-11 Symbiotic pseudorevertant of MYa179 This study
SRM-12 Symbiotic pseudorevertant of MYa179 This study
E. coli
MM294A pro-82 thi-1 endA hsdR17 supE44 Glazebrook & Walker (1991)
Plasmids
pRK609 pRK600 {Omega}TnphoA Long et al. (1988)
pFAJ1702 Tcr, broad-host-range cloning vector Dombrecht et al. (2001)
pYK33 pFAJ1702 containing 2.1 kb cep+ fragment This study
pAK37 pFAJ1702 containing lacZ fused to groES1 promoter This study
pRK600 pRK2013 Nmr : : Tn9, RK2 tra genes Finan et al. (1986)
pHP4 {Omega} {Omega} interposon (Smr/Spr gene cassette) Prentki & Krisch (1984)
pMS266 Gmr gene cassette Becker et al. (1995)
pK18mob Suicide vector, Nmr Schäfer et al. (1994)