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Journal of General Microbiology 113 (1979), 369-375; DOI  10.1099/00221287-113-2-369
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Microbial Determinations by Flow Cytometry

K.-J. HUTTER{ddagger},* and H. E. EIPEL{dagger}

* Fraunhofer Gesellschaft, Institut für Aerobiologie, D-5948 Schmallenberg, Germany
{dagger} Biophysics Systems GmbH, Im Eichenböhl 24, D-6140 Bensheim 3, Germany

ABSTRACT

Summary: Recent improvements in the optics and electronics of flow cytometry systems, as well as in staining techniques, permit the assay of such minute cellular constituents as the DNA and protein contents of micro-organisms. To assess the usefulness of this technique, DNA and protein content distributions were determined in Escherichia coli, Lactobacillus brevis, Lactobacillus casei, Chlorella kessleri 8k, Saccharomyces cerevisiae, Candida utilis, Schizosaccharomyces pombe and Euglena gracilis.Investigations of the DNA content distributions of polyploid strains of Saccharomyces cerevisiae indicated that the method can be used to determine ploidy. The rapidity of flow cytometry measurements allows accurate determinations in large populations.

{ddagger} Present address: Deutsches Krebsforschungszentrum Heidelberg, Institut für experimentelle Pathologie, Im Neuenheimer Feld 280, D-6900 Heidelberg 1, Germany.




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