HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Garrett, J. M. Search for Related Content PubMed PubMed Citation Articles by Garrett, J. M. Agricola Articles by Garrett, J. M.

Characterization of AAT1: a Gene Involved in the Regulation of Amino Acid Transport in Saccharomyces cerevisiae

Department of Biology, Hamilton College, Clinton, New York 13323, USA

ABSTRACT

SUMMARY: A new class of Saccharomyces cerevisiae mutants (aatl - amino acid transport) has been identified. These mutants are unable to grow on rich medium or on minimal medium supplemented with certain amino acids (isoleucine, methionine, phenylalanine, tyrosine or valine). This phenotype is directly linked to the presence of the leu2 allele in these strains: aat1 LEU2 organisms grow normally on all media tested. Leucine uptake through the leucine-specific permease is inhibited to < 35% of wild-type levels in aat1 cells preincubated in nonpermissive media, and the activity of the general amino acid permease is also low in these conditions, aat1 cells are therefore unable to grow on rich media because they cannot take up enough leucine to supplement their auxotrophic requirement.

This article has been cited by other articles:

				P Martinez and P. Ljungdahl The SHR3 homologue from S. pombe demonstrates a conserved function of ER packaging chaperones J. Cell Sci., January 12, 2000; 113(23): 4351 - 4362. [Abstract] [PDF]

				H. Klasson, G. R. Fink, and P. O. Ljungdahl Ssy1p and Ptr3p Are Plasma Membrane Components of a Yeast System That Senses Extracellular Amino Acids Mol. Cell. Biol., August 1, 1999; 19(8): 5405 - 5416. [Abstract] [Full Text] [PDF]