HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Marrec, C. L. Articles by Trautwetter, A. Search for Related Content PubMed Articles by Marrec, C. L. Articles by Trautwetter, A. Agricola Articles by Marrec, C. L. Articles by Trautwetter, A.

áU2, a temperate bacteriophage specific for ‘Arthrobacter aureus’, whose integrative functions work in other corynebacteria

Laboratoire de Génétique et Physiologie Microbiennes, CNRS URA 256, Université de Rennes I, Campus de Beaulieu, UFR-SVE, Av. du Général Leclerc, F-35042 Rennes Cedex, France

Author for correspondence: Annie Trauwetter. Tel: +33 99 28 61 40. Fax: +33 99 28 67 00.

ABSTRACT

The temperate bacteriophage AAU2 of ‘Arthrobacter aureus’ C70, isolated from a soil sample, has been purified and characterized. The phage belongs morphologically to the Siphoviridae family. Its genome consists of a linear double-stranded DNA of 45.4 kb with cohesive ends. The attP site and the phage-encoded functions essential for integration were cloned in Escherichia coli as a 5.25 kb BglII-EcoRV fragment. The resulting plasmid vector, non-replicating in ‘A. aureus’, is able to integrate at high frequency into the chromosomes of ‘A. aureus’ and the industrially used coryneform bacteria Brevibacterium lactofermentum and Corynebacterium glutamicum.

This article has been cited by other articles:

				K. Gouffi, N. Pica, V. Pichereau, and C. Blanco Disaccharides as a New Class of Nonaccumulated Osmoprotectants for Sinorhizobium meliloti Appl. Envir. Microbiol., April 1, 1999; 65(4): 1491 - 1500. [Abstract] [Full Text]