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1Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
2Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06032, USA
Author for correspondence: Anne Moir. Tel: +44 742 824418. Fax: +44 742 72869. e-mail:a.moir@uk.ac.sheffield
ABSTRACT
The gerB spore germination operon of Bacillus subtilis 168 is a homologue of the gerA spore germination operon. The expression and regulation of the gerB operon has been examined using a lacZ transcriptional fusion and the transcriptional start defined. The gerB operon is expressed during sporulation under the control of RNA polymerase containing the forespore-specific sigma factor,
G. This is a further homology to the gerA operon, which is similarly regulated. It is predicted from the localization of expression and the encoded primary sequences that the GerB proteins are located at the inner spore membrane.
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