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Microbiology 140 (1994), 425-431; DOI  10.1099/13500872-140-2-425
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The linear-plasmid-encoded toxin produced the yeast Pichia acaciae: characterization an comparison with the toxin of Kluyveromyce: lactis

Derek A. McCracken1, Vera J. Martin2, Michael J. R. Stark2 and Paul L. Bolen3,{dagger}

Department of Biological Sciences, Illinois State University, Normal, IL 61790-4120, USA
Department of Biochemistry, The University, Dundee DD1 4HN, UK
National Center for Agricultural Utilization Research, USDA, Peoria, IL 61604, USA

ABSTRACT

Summary: The toxin produced by Pichia acaciae was purified and its properties comp to those of the toxin from Kluyveromyces lactis. Like this toxin, the P. aca toxin is a protein comprised of three subunits (molecular masses 110, 39 a 38 kDa) with an associated chitinase activity and a pH optimum between 7.0 and 7.5. P. acaciae toxin also caused G1 cell cycle arrest. Of the thirteen recessive alleles that provided resistance in Saccharomyces cerevisiae to A lactis toxin, only three also conferred resistance to P. acaciae toxin. Simila and differences in the interactions of the two toxins with yeast cells are discussed.

Author for correspondence: Derek A. McCracken. Tel: +1 309 438 7689. Fax: +1 309 438 3722.

Keywords: yeast, Pichia acaciae toxin, cell cycle arrest

{dagger} Present address: International Flavors and Fragrances Inc., Corporate Research and Development Centre, Union Beach, NJ 07735, USA.




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