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Microbiology 140 (1994), 697-702
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microbiology, Vol 140, 697-702, Copyright © 1994 by Society for General Microbiology

ARTICLES

Pediocin A, a bacteriocin produced by Pediococcus pentosaceus FBB61

A Piva and DR Headon
Istituto di Zootecnia e Nutrizione Animale, Facolta di Medicina Veterinaria, Universita di Bologna, Ozzano Emilia (BO), Italy.

Pediocin A, a bacteriocin produced by Pediococcus pentosaceus FBB61, was purified and partially characterized. The purification was achieved by dialysis against PEG 20,000, butanol extraction and electroendosmotic preparative electrophoresis. The protocol led to a 7843-fold increase in the specific activity, with 3.9% activity recovered. SDS-PAGE of pediocin A resulted in a single 80 kDa protein band. The antimicrobial compound was sensitive to proteolytic enzymes and heat (10 min at 100 degrees C). It exhibited inhibition against species of Lactobacillus, Lactococcus, Leuconostoc, Pediococcus, Staphylococcus, Enterococcus, Listeria and Clostridium.


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