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Composition of 2,3-dihydroxy fatty acid-containing lipopolysaccharides from Legionella israelensis, Legionella maceachernii and Legionella micdadei

Anders Sonesson^1,*,

National Institute of Public Health, Geitmyrsveien 75, N-0462 Oslo, Norway
Forschungsinstitut Borstel, Parkallee 22, D-23845 Borstel, Germany

^*Author for correspondence: Anders Sonesson. Tel: +1 615 974 8014. Fax: +1 615 974 8086.

ABSTRACT

Lipopolysaccharides (LPS) from Legionella israelensis, L. maceachernii and L. micdadei were analysed for chemical composition. The main sugar of the lipid A fractions was in each case 2,3-diamino-2,3-dideoxy-D-glucose. Lipid A of L. israelensis also contained a substantial amount of D-glucosamine. In each lipid A fraction a complex fatty acid pattern was detected. This comprised at least 19 different 3-hydroxy fatty acids (amide-linked), three 2,3-dihydroxy fatty acids (amide-linked), non-hydroxy fatty acids (ester-linked) as well as long-chain (-1)-oxo, (-1)-hydroxy and (1, )-dioic fatty acids (ester-linked). In addition, L. maceachernii and L. micdadei contained -hydroxylated long-chain (-1)-oxo and (1, )-dioic fatty acids. The polysaccharide parts of L. maceachernii and L. micdadei LPS were similar and contained mainly L-rhamnose, L-fucose, D-mannose, D-glucose, L-fucosamine, D-glucosamine, 2-keto-3-deoxy-octonic acid (Kdo) as well as the rare octose yersiniose A. The corresponding composition of L. israelensis LPS was simpler and consisted mainly of L-rhamnose and 3-amino-3,6-dideoxy-D-mannose. LPS of L. israelensis and L. micdadei contained, in addition, 2-keto-octonic acid linked to Kdo. Phosphorylated sugar constituents were detected in all three LPS, whereas ethanolamine was found only in LPS from L. maceachernii. The SDS-PAGE band pattern of L. micdadei differed from the two others in a higher proportion of the low molecular mass constituents.

Present address: Center for Environmental Biotechnology, Uniersity of Tennessee, 105 15 Research Drive, Suite 300, Knoxville, TN 37932–2567, USA.

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