Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Microbiology 140 (1994), 1285-1290
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by O'Rourke, M.
Right arrow Articles by Spratt, B. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by O'Rourke, M.
Right arrow Articles by Spratt, B. G.
Agricola
Right arrow Articles by O'Rourke, M.
Right arrow Articles by Spratt, B. G.

microbiology, Vol 140, 1285-1290, Copyright © 1994 by Society for General Microbiology

ARTICLES

Further evidence for the non-clonal population structure of Neisseria gonorrhoeae: extensive genetic diversity within isolates of the same electrophoretic type

M O'Rourke and BG Spratt
Microbial Genetics Group, School of Biological Sciences, University of Sussex, Falmer, Brighton, UK.

Analysis of data from multilocus enzyme electrophoresis has revealed that Neisseria gonorrhoeae populations are non-clonal. Fifteen percent of 227 isolates of N. gonorrhoeae had an identical multilocus genotype (ET1) and were recovered world-wide over a 26 year period. The recovery of isolates of identical multilocus genotype from geographically and temporally unassociated hosts is a common criterion of a clonal population structure. However, in a recombining (non-clonal) population, isolates with the same multilocus genotype can arise by the random association of the most common alleles in the population. Analysis of the variation in two further enzymes, in the restriction patterns obtained from the glutamine synthetase gene, and in the DNA fragments obtained using an arbitrarily primed polymerase chain reaction, was used to show that members of ET1 were almost as variable as randomly selected N. gonorrhoeae isolates. Unlike the situation in a strongly clonal species, the 26 ET1 isolates examined were increasingly sub-divided to give 19 distinguishable groups as variation at further loci was examined, and 24 distinguishable groups when auxotypes were also considered. We conclude that, as expected of a non-clonal population, the most commonly encountered N. gonorrhoeae multilocus genotype does not define a clone.


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
L. E. Garvin, M. C. Bash, C. Keys, D. M. Warner, S. Ram, W. M. Shafer, and A. E. Jerse
Phenotypic and Genotypic Analyses of Neisseria gonorrhoeae Isolates That Express Frequently Recovered PorB PIA Variable Region Types Suggest that Certain P1a Porin Sequences Confer a Selective Advantage for Urogenital Tract Infection
Infect. Immun., August 1, 2008; 76(8): 3700 - 3709.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
I. M. C. Martin, A. Ghani, G. Bell, G. Kinghorn, and C. A. Ison
Persistence of Two Genotypes of Neisseria gonorrhoeae during Transmission
J. Clin. Microbiol., December 1, 2003; 41(12): 5609 - 5614.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
M. Hobbs, B Malorny, P Prasad, G Morelli, B Kusecek, J. Heckels, J. Cannon, and M Achtman
Recombinational reassortment among opa genes from ET-37 complex Neisseria meningitidis isolates of diverse geographical origins
Microbiology, January 1, 1998; 144(1): 157 - 166.
[Abstract]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1994 Society for General Microbiology.