Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Microbiology 140 (1994), 1301-1305; DOI  10.1099/00221287-140-6-1301
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Swindell, S. R.
Right arrow Articles by Gasson, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Swindell, S. R.
Right arrow Articles by Gasson, M. J.
Agricola
Right arrow Articles by Swindell, S. R.
Right arrow Articles by Gasson, M. J.

Cloning, sequencing and comparison of three lactococcal L-lactate dehydrogenase genes

Simon R. Swindell1,*, Hugh G. Griffin1 and Michael J. Gasson1

AFRC Institute of Food Research, Norwich Laboratory, Norwich Research Park, Colney, Norwich NR4 7UA, UK

*Author for correspondence: Simon R. Swindell. Tel: +44 603 255177. Fax: 603 507723. e-mail: Swindell@AFRC.AC.UK

ABSTRACT

The conversion of pyruvate to lactate is a key feature of lactococcal strains. The enzyme which facilitates this conversion, L-lactate dehydrogenase (LDH), and the gene which encodes it (Idh), are therefore of great significance. This paper presents the cloning and DNA sequence analysis of three further lactococcal genes which are of key importance in the genetic manipulation of commercial starter strains. The Idh gene from Lactococcus lactis subsp. lactis biovar diacetylactis BU2-60 has been isolated from a lambda library and sequenced. The Idh gene from L. lactis subsp. cremoris NCDO 762 and that from L. lactis subsp. lactis IL1403 have been amplified by the polymerase chain reaction (PCR) and sequenced. These DNA sequences and deduced amino acid sequences have been compared with those from L. lactis subsp. lactis MG1363. The LDHs from L. lactis subsp. lactis MG1363 and L. lactis subsp. cremoris NCDO 762 are 99.4% homologous. The LDHs from L. lactis subsp. lactis MG1363 and L. lactis subsp. lactis IL1403 are 96.4% homologous. The LDHs from L. lactis subsp. lactis IL1403 and L. lactis subsp. lactis biovar diacetylactis BU2-60 are 99,9% homologous. Our results provide further evidence that L. lactis subsp. lactis MG1363 and other L. lactis subsp. lactis NCDO 712 derived strains should be reclassified as Lactococcus lactis subsp. cremoris

Keywords: lactate dehydrogenase gene, lactococci, sequencing, classification




This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
E. W. J. van Niel, J. Palmfeldt, R. Martin, M. Paese, and B. Hahn-Hagerdal
Reappraisal of the Regulation of Lactococcal L-Lactate Dehydrogenase
Appl. Envir. Microbiol., March 1, 2004; 70(3): 1843 - 1846.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
M. Nomura, M. Kobayashi, and T. Okamoto
Rapid PCR-Based Method Which Can Determine Both Phenotype and Genotype of Lactococcus lactis Subspecies
Appl. Envir. Microbiol., May 1, 2002; 68(5): 2209 - 2213.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
M. Nomura, M. Kobayashi, S. Ohmomo, and T. Okamoto
Inactivation of the Glutamate Decarboxylase Gene in Lactococcus lactis subsp. cremoris
Appl. Envir. Microbiol., May 1, 2000; 66(5): 2235 - 2237.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1994 Society for General Microbiology.