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Microbiology 140 (1994), 1337-1340
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microbiology, Vol 140, 1337-1340, Copyright © 1994 by Society for General Microbiology

ARTICLES

The expression of the bstVIM gene from Bacillus stearothermophilus V is restricted to vegetative cell growth

E Gonzalez, C Padilla, C Saavedra and C Vasquez
Laboratorio de Biologia Molecular, Universidad de Talca, Chile.

The activity of BstVI DNA methyltransferase was monitored during the sporulative cycle of Bacillus stearothermophilus V. Significant methylase activity was found only in bacteria growing vegetatively. This was confirmed by Northern hybridization, which indicated that the bstVIM gene was not transcribed in cells undergoing sporulation. Supporting evidence came from experiments which demonstrated that the RNA polymerase holoenzyme from these cells did not recognize the promoter elements upstream of the bstVIM gene.




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