Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Microbiology 140 (1994), 1341-1350; DOI  10.1099/00221287-140-6-1341
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thaller, M. C.
Right arrow Articles by Rossolini, G. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thaller, M. C.
Right arrow Articles by Rossolini, G. M.
Agricola
Right arrow Articles by Thaller, M. C.
Right arrow Articles by Rossolini, G. M.

Characterization and sequence of PhoC, the principal phosphate-irrepressible acid phosphatase of Morganella morganii

Maria Cristina Thaller1, Francesca Berlutti1, Serena Schippa1, Giovanna Lombardi2 and Gian Maria Rossolini2,*

Istituto di Microbiologia, Università ‘La Sapienza’, Piazzale A. Moro, 5, 00185-Rome, Italy
Dipartimento di Biologia Molecolare - Sezione di Microbiologia, Università di Siena, Via Laterina, 8, 53100-Siena, Italy

*Author for correspondence: G. M. Rossolini, Tel: +39 577 280903. Fax: + 39 577 42011.

ABSTRACT

Phosphatase activities were investigated in Morganella morganii, which is one of the few enterobacterial species producing high-level phosphateirrepressible acid phosphatase activity (HPAP phenotype), and the gene encoding the major phosphate-irrepressible acid phosphatase was cloned, sequenced, and its product characterized. Using p-nitrophenyl phosphate as substrate, Morganella produced a major phosphate-irrepressible acid phosphatase (named PhoC) which is associated with the HPAP phenotype, a minor phosphate-irrepressible acid phosphatase, and a phosphate-repressible alkaline phosphatase. The presence of the PhoC activity prevented induction of alkaline phosphatase when a PhoC-hydrolysable organic phosphate ester, such as glycerol 2-phosphate, was the sole phosphate source. PhoC is a secreted nonspecific acid phosphatase apparently composed of four 25 kDa polypeptide subunits. The enzyme is resistant to EDTA, Pi, fluoride and tartrate. The M. morganii PhoC showed 84.6% amino acid sequence identity to the PhoN nonspecific acid phosphatase of Providencia stuartii, 45.3 % to the PhoN nonspecific acid phosphatase of Salmonella typhimurium, and 37.8% to the principal acid phosphatase (PhoC) of Zymomonas mobilis. Comparison of sequence data and of regulation of these enzymes suggested a different phylogeny of members of this gene family within the Enterobacteriaceae.

Keywords: Morganella morganii, PhoC, phosphatase activities




This article has been cited by other articles:


Home page
 MicrobiologyHome page
R. Wang, K. Ohtani, Y. Wang, Y. Yuan, S. Hassan, and T. Shimizu
Genetic and biochemical analysis of a class C non-specific acid phosphatase (NSAP) of Clostridium perfringens
Microbiology, January 1, 2010; 156(1): 167 - 173.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Bernard, M. El Ghachi, D. Mengin-Lecreulx, M. Chippaux, and F. Denizot
BcrC from Bacillus subtilis Acts as an Undecaprenyl Pyrophosphate Phosphatase in Bacitracin Resistance
J. Biol. Chem., August 12, 2005; 280(32): 28852 - 28857.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
D. Serruto and C. L. Galeotti
The signal peptide sequence of a lytic transglycosylase of Neisseria meningitidis is involved in regulation of gene expression
Microbiology, May 1, 2004; 150(5): 1427 - 1437.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
F. Berlutti, C. Passariello, L. Selan, M. C. Thaller, and G. M. Rossolini
The Chryseobacterium meningosepticum PafA enzyme: prototype of a new enzyme family of prokaryotic phosphate-irrepressible alkaline phosphatases?
Microbiology, October 1, 2001; 147(10): 2831 - 2839.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
Y. Mihara, T. Utagawa, H. Yamada, and Y. Asano
Phosphorylation of Nucleosides by the Mutated Acid Phosphatase from Morganella morganii
Appl. Envir. Microbiol., July 1, 2000; 66(7): 2811 - 2816.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
X. Chen, T. Ansai, S. Awano, T. Iida, S. Barik, and T. Takehara
Isolation, Cloning, and Expression of an Acid Phosphatase Containing Phosphotyrosyl Phosphatase Activity from Prevotella intermedia
J. Bacteriol., November 15, 1999; 181(22): 7107 - 7114.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
F. Berlutti, M. Casalino, C. Zagaglia, P. A. Fradiani, P. Visca, and M. Nicoletti
Expression of the Virulence Plasmid-Carried Apyrase Gene (apy) of Enteroinvasive Escherichia coli and Shigella flexneri Is under the Control of H-NS and the VirF and VirB Regulatory Cascade
Infect. Immun., October 1, 1998; 66(10): 4957 - 4964.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
W. Hemrika, R. Renirie, H. L. Dekker, P. Barnett, and R. Wever
From phosphatases to vanadium peroxidases: A similar architecture of the active site
PNAS, March 18, 1997; 94(6): 2145 - 2149.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1994 Society for General Microbiology.