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Microbiology 140 (1994), 1373-1375; DOI  10.1099/00221287-140-6-1373
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Electrophoretic karyotype of Fusarium solani

Sarita W. Nazareth1,{dagger} and Carlo V. Bruschi1,*

Department of Microbiology, International Centre for Genetic Engineering and Biotechnology, Padriciano 99, 1-34012 ltaly

*Author for correspondence: Carlo V. Bruschi. Tel: + 39 40 375 7343. e-mail: bruschi@icgeb.trieste.it

ABSTRACT

The electrophoretic karyotype of Fusarium solani was determined by contour-clamped homogeneous electric field (CHEF) gel electrophoresis. Thirteen chromosomal bands were obtained. The size of these bands, based on their migration relative to the chromosomal DNA of Schizosaccharomyces pombe and Saccharomyces cerevisiae, is estimated to be between 0.42 and 6.08 Mb, the total molecular size of the DNA being 39.90 Mb.


Keywords: Fusarium solani, electrophoretic karyotype, CHEF, chromosomes

{dagger} Present address: Department of Microbiology, Goa University, Taleigao Plateau, Bambolim, Goa-403202, India.







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