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Microbiology 141 (1995), 141-146
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microbiology, Vol 141, 141-146, Copyright © 1995 by Society for General Microbiology

ARTICLES

Cloning and molecular analysis of the Salmonella enterica ansP gene, encoding an L-asparagine permease

MP Jennings, JK Anderson and IR Beacham
Division of Science and Technology, Griffith University, Nathan, Queensland, Australia.

A gene (ansP), which encodes an L-asparagine permease, has been isolated from a cosmid library of Salmonella enterica during screening for recombinant clones which encode L-asparaginase. Nucleotide sequence analysis reveals that the gene product is a polypeptide of 497 amino acid residues, containing 12 putative transmembrane segments. The calculated molecular mass is 54 kDa, although maxicell analysis by SDS- PAGE gave an apparent molecular mass of 37 kDa. Comparison of the deduced amino acid sequence with sequence databases showed significant homology with a family of basic and aromatic amino acid permeases. Strains containing the cloned ansP gene demonstrated a many-fold increase in L-asparagine uptake in comparison with control strains.




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