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Microbiology 141 (1995), 2673-2679
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microbiology, Vol 141, 2673-2679, Copyright © 1995 by Society for General Microbiology

ARTICLES

Isolation of canCHS1A, a variant gene of Candida albicans chitin synthase

M Sudoh, M Watanabe, T Mio, S Nagahashi, H Yamada-Okabe, M Takagi and M Arisawa
Department of Mycology, Nippon Roche Research Center, Kanagawa, Japan.

A canCHS1A gene encoding the chitin synthase of Candida albicans was cloned. DNA sequencing and comparison with another canCHS1 gene described elsewhere indicated that the canCHS1A gene encoded a polypeptide with 775 amino acid residues, a protein with one less amino acid than that encoded by the canCHS1 gene. A six-base alteration was observed between the two genes, suggesting that the canCHS1A gene is a variant gene of the canCHS1. The pH profile/activity relationship of canChs1A in permeabilized cells was identical to that of canChs1. The canChs1A enzyme was competitively inhibited by polyoxin D (5.2 microM) and nikkomycin Z (12 microM). When the cloned gene was expressed in a Saccharomyces cerevisiae chs2 mutant that exhibited aberrant morphology, the normal structure was restored. We conclude that the function of the canCHS1A gene is similar to that of sacCHS2 in S. cerevisiae.


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M. Sudoh, T. Yamazaki, K. Masubuchi, M. Taniguchi, N. Shimma, M. Arisawa, and H. Yamada-Okabe
Identification of a Novel Inhibitor Specific to the Fungal Chitin Synthase. INHIBITION OF CHITIN SYNTHASE 1 ARRESTS THE CELL GROWTH, BUT INHIBITION OF CHITIN SYNTHASE 1 AND 2 IS LETHAL IN THE PATHOGENIC FUNGUS CANDIDA ALBICANS
J. Biol. Chem., October 13, 2000; 275(42): 32901 - 32905.
[Abstract] [Full Text] [PDF]


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