microbiology, Vol 141, 583-588, Copyright © 1995 by Society for General Microbiology

ARTICLES

Synthesis of glycerophosphorylated cyclic (1,2)-beta-glucans in Rhizobium meliloti strain 1021 after osmotic shock

MW Breedveld and KJ Miller
Department of Food Science, Pennsylvania State University, University Park 16802.

The transfer of phosphoglycerol moieties from phosphatidylglycerol to the cyclic (1,2)-beta-glucans in growing cultures of Rhizobium meliloti strain 1021 was investigated using pulse-chase experiments with [3H]glycerol and/or [14C]glucose. No transfer occurred when cells were grown and pulse-chased in a medium containing 0.4 M NaCl. However, radiolabelled glycerophosphorylated cyclic (1,2)-beta-glucans could be detected within 30 min after transfer of these cultures to a low- osmolarity medium. Conversely, when low-osmolarity cultures were shifted to a high-osmolarity medium containing 0.4 M NaCl or 0.8 M sucrose, the transfer of phosphoglycerol substituents to the cyclic (1,2)-beta-glucans was inhibited. Further experiments revealed that the transfer of phosphoglycerol substituents to the cyclic (1,2)-beta- glucans occurs within the periplasmic compartment.

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