microbiology, Vol 141, 1937-1945, Copyright © 1995 by Society for General Microbiology

ARTICLES

I-CeuI recognition sites in the rrn operons of the Bacillus subtilis 168 chromosome: inherent landmarks for genome analysis

T Toda and M Itaya
School of Science and Engineering, Saitama University, Japan.

The Bacillus subtilis 168 circular chromosome yielded ten fragments on I-CeuI endonuclease digestion. I-CeuI recognizes a 26 bp sequence that is located within the gene encoding the 23S subunit of the rRNA in Chlamydomonas eugametos, Escherichia coli and Salmonella typhimurium. The precise locations of the I-CeuI sites of the B. subtilis chromosome were determined on a NotI-SfiI physical map by (i) double digestion analyses with I-CeuI and SfiI, (ii) comparison of mutant strains lacking a specific rrn operon, (iii) using an I-CeuI linking clone and (iv) analysis of nucleotide sequence data of some rrn operons. In conclusion, all the I-CeuI sites were located within the B. subtilis rrn operons and the I-CeuI sites were conserved in all the B. subtilis 168 derivatives tested. Thus, variations in size of the I-CeuI fragments must be due to genome alterations. A B. subtilis 168 strain was investigated with I-CeuI. We demonstrated that the aberrant structure was the outcome of the inversion of an approximately 1700 kb DNA segment.

This article has been cited by other articles:

				A. Kuroki, T. Toda, K. Matsui, R. Uotsu-Tomita, M. Tomita, and M. Itaya Reshuffling of the Bacillus subtilis 168 Genome by Multifold Inversion J. Biochem., January 1, 2008; 143(1): 97 - 105. [Abstract] [Full Text] [PDF]

				M. Itaya, K. Tsuge, M. Koizumi, and K. Fujita From The Cover: Combining two genomes in one cell: Stable cloning of the Synechocystis PCC6803 genome in the Bacillus subtilis 168 genome PNAS, November 1, 2005; 102(44): 15971 - 15976. [Abstract] [Full Text] [PDF]

				D. Qiu, K. Fujita, Y. Sakuma, T. Tanaka, Y. Ohashi, H. Ohshima, M. Tomita, and M. Itaya Comparative Analysis of Physical Maps of Four Bacillus subtilis (natto) Genomes Appl. Envir. Microbiol., October 1, 2004; 70(10): 6247 - 6256. [Abstract] [Full Text] [PDF]

				P. Le Bourgeois, M.-L. Daveran-Mingot, and P. Ritzenthaler Genome Plasticity among Related Lactococcus Strains: Identification of Genetic Events Associated with Macrorestriction Polymorphisms J. Bacteriol., May 1, 2000; 182(9): 2481 - 2491. [Abstract] [Full Text]

				A. C. Padovan, G. Firrao, B. Schneider, and K. S. Gibb Chromosome mapping of the sweet potato little leaf phytoplasma reveals genome heterogeneity within the phytoplasmas Microbiology, April 1, 2000; 146(4): 893 - 902. [Abstract] [Full Text]

				M. L. Hunt, C. G. Ruffolo, K. Rajakumar, and B. Adler Physical and Genetic Map of the Pasteurella multocida A:1 Chromosome J. Bacteriol., November 15, 1998; 180(22): 6054 - 6058. [Abstract] [Full Text]

				M.-L. Daveran-Mingot, N. Campo, P. Ritzenthaler, and P. Le Bourgeois A Natural Large Chromosomal Inversion in Lactococcus lactis Is Mediated by Homologous Recombination between Two Insertion Sequences J. Bacteriol., September 15, 1998; 180(18): 4834 - 4842. [Abstract] [Full Text]

				M. Itaya and T. Tanaka Experimental surgery to create subgenomes of Bacillus subtilis 168 PNAS, May 13, 1997; 94(10): 5378 - 5382. [Abstract] [Full Text] [PDF]