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Department of Molecular Biology, Faculty of Science, Nagoya University, Chikusa-Ku, Nagoya 464-01, Japan
1Author for correspondence: Michio Homma. Tel: +81 52 789 2992. Fax: +81 52 789 3001. e-mail: g44416a@nucc.cc.nagoya-u.ac.ip
ABSTRACT
Chemotactic responses in Vibrio alginolyticus, which has lateral and polar flagellar systems in one cell, were investigated. A lateral-flagella-defective (Pof- Laf-) mutant, which has only a polar flagellum, usually swam forward by the pushing action of its flagellum and occasionally changed direction by backward swimming. When the repellent phenol was added, Pof+ Laf- cells moved frequently forward and backward (tumbling state). The tumbling was derived from the frequent changing between counter-clockwise and clockwise (CW) rotation of the flagellar motor, as was confirmed by the tethered-cell method. Furthermore, we found that the tumbling cells did not adapt to the phenol stimulus. When the attractant serine was added, the phenol-treated cells ceased tumbling and swam smoothly, adapting to the attractant stimulus after several minutes. We isolated chemotaxis-defective (Che-) mutants from the Pof+ Laf- mutant; the tumbling mutants were not isolated. One interesting mutant swam backwards continuously, with its flagellum leading the cell and its flagellar motor rotating CW continuously. A polar-flagella-defective mutant (Pof- Laf+) stopped swimming after phenol addition and then recovered swimming ability within 10 min, indicating that lateral flagella can adapt to the repellent stimulus. This may represent a functional difference between the two flagellar systems in Vibrio cells, and between the chemotaxis systems affecting the two types of flagella.
Present address: Professor Yasuo Imae died suddenly of a cerebral haemorrhage on 2 July 1993. This article is dedicated to him.
This article has been cited by other articles:
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K. Kogure, E. Ikemoto, and H. Morisaki Attachment of Vibrio alginolyticus to Glass Surfaces Is Dependent on Swimming Speed J. Bacteriol., February 15, 1998; 180(4): 932 - 937. [Abstract] [Full Text]
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