microbiology, Vol 142, 3047-3056, Copyright © 1996 by Society for General Microbiology

ARTICLES

The 25 degrees-36 degrees region of the Bacillus subtilis chromosome: determination of the sequence of a 146 kb segment and identification of 113 genes

K Yamane, M Kumano and K Kurita
Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan. kyamane@sakura.cc.tsukuba.ac.jp

We determined a 146 kb contiguous sequence at the 25 degrees-36 degrees region of the Bacillus subtilis chromosome containing the amyE-srfA segment. Among the 113 ORFs identified, 33 are already known. functions were assigned to 38 ORFs by a search of non-redundant protein sequence data banks and those of 16 ORFs were suggested through significant similarity with reported sequences. The amino acid sequences of 13 of the ORfs were similar to proteins of unknown function of Escherichia coli, Haemophilus influenzae and other species. We did not find similarities for 29 ORFs to any known proteins. The 146 kb region is rich in enzymes (35 ORFs) related to the metabolism of low molecular mass compounds and five genes for surfactin production occupy about 26 kb of the region.

This article has been cited by other articles:

				J. A. Wouters, T. Hain, A. Darji, E. Hufner, H. Wemekamp-Kamphuis, T. Chakraborty, and T. Abee Identification and Characterization of Di- and Tripeptide Transporter DtpT of Listeria monocytogenes EGD-e Appl. Envir. Microbiol., October 1, 2005; 71(10): 5771 - 5778. [Abstract] [Full Text] [PDF]

				R. Ohki and K. Tateno Increased Stability of bmr3 mRNA Results in a Multidrug-Resistant Phenotype in Bacillus subtilis J. Bacteriol., November 1, 2004; 186(21): 7450 - 7455. [Abstract] [Full Text] [PDF]

				L. Barthelmebs, C. Divies, and J.-F. Cavin Knockout of the p-Coumarate Decarboxylase Gene from Lactobacillus plantarum Reveals the Existence of Two Other Inducible Enzymatic Activities Involved in Phenolic Acid Metabolism Appl. Envir. Microbiol., August 1, 2000; 66(8): 3368 - 3375. [Abstract] [Full Text]

				A. L. Honeyman and R. Curtiss III The mannitol-specific enzyme II (mtlA) gene and the mtlR gene of the PTS of Streptococcus mutans Microbiology, July 1, 2000; 146(7): 1565 - 1572. [Abstract] [Full Text]

				H. Cruz Ramos, T. Hoffmann, M. Marino, H. Nedjari, E. Presecan-Siedel, O. Dreesen, P. Glaser, and D. Jahn Fermentative Metabolism of Bacillus subtilis: Physiology and Regulation of Gene Expression J. Bacteriol., June 1, 2000; 182(11): 3072 - 3080. [Abstract] [Full Text]

				S. Vílchez, L. Molina, C. Ramos, and J. L. Ramos Proline Catabolism by Pseudomonas putida: Cloning, Characterization, and Expression of the put Genes in the Presence of Root Exudates J. Bacteriol., January 1, 2000; 182(1): 91 - 99. [Abstract] [Full Text]

				H. Fiegler, J. Bassias, I. Jankovic, and R. Brückner Identification of a Gene in Staphylococcus xylosus Encoding a Novel Glucose Uptake Protein J. Bacteriol., August 15, 1999; 181(16): 4929 - 4936. [Abstract] [Full Text]

				J. Huang, 2. He, and J. Wiegel Cloning, Characterization, and Expression of a Novel Gene Encoding a Reversible 4-Hydroxybenzoate Decarboxylase from Clostridium hydroxybenzoicum J. Bacteriol., August 15, 1999; 181(16): 5119 - 5122. [Abstract] [Full Text]

				H. E. Smith, M. Damman, J. van der Velde, F. Wagenaar, H. J. Wisselink, N. Stockhofe-Zurwieden, and M. A. Smits Identification and Characterization of the cps Locus of Streptococcus suis Serotype 2: the Capsule Protects against Phagocytosis and Is an Important Virulence Factor Infect. Immun., April 1, 1999; 67(4): 1750 - 1756. [Abstract] [Full Text] [PDF]

				H. Tjalsma, V. P. Kontinen, Z. Pragai, H. Wu, R. Meima, G. Venema, S. Bron, M. Sarvas, and J. M. van Dijl The Role of Lipoprotein Processing by Signal Peptidase II in the Gram-positive Eubacterium Bacillus subtilis. SIGNAL PEPTIDASE II IS REQUIRED FOR THE EFFICIENT SECRETION OF alpha -AMYLASE, A NON-LIPOPROTEIN J. Biol. Chem., January 15, 1999; 274(3): 1698 - 1707. [Abstract] [Full Text] [PDF]