microbiology, Vol 142, 3113-3123, Copyright © 1996 by Society for General Microbiology

ARTICLES

Sequencing of a 65 kb region of the Bacillus subtilis genome containing the lic and cel loci, and creation of a 177 kb contig covering the gnt- sacXY region

K Yoshida, K Shindo, H Sano, S Seki, M Fujimura, N Yanai, Y Miwa and Y Fujita
Department of Biotechnology, Faculty of Engineering, Fukuyama University, Hiroshima, Japan.

Within the framework of an international project for the sequencing of the entire Bacillus subtilis genome, this paper communicates the sequencing of a chromosome region containing the lic and cel loci (65 kb), which creates a 177 kb contig covering the region from gnt to sacXY. This 65 kb region contains 64 ORFs (62 complete and two partial genes). The 14th, 15th and 17th genes correspond to licT, licS and katE, encoding the antiterminator for licS transcription, beta- glucanase (lichenase) and catalase 2, respectively. The 11th, 30th, 36th, 39th, 41st, 45th-48th, 51st and 58th genes are designated deaD, pepT, galE, aldY, msmX, cydABCD, sigY and katX because their products probably encode ATP-dependent RNA helicase, tripeptidase, UDP-glucose 4- epimerase, aldehyde dehydrogenase, multiple sugar-binding transport ATP- binding protein, the respective components of cytochrome d ubiquinol oxidase and ATP-binding cassette transporter, sigma-factor of RNA polymerase and catalase, respectively. The 60th-64th genes are celRABCD, which are probably involved in cellobiose utilization. Gene organization and gene features in the gnt-sacXY region are discussed.

This article has been cited by other articles:

				M. Yoshimura, K. Asai, Y. Sadaie, and H. Yoshikawa Interaction of Bacillus subtilis extracytoplasmic function (ECF) sigma factors with the N-terminal regions of their potential anti-sigma factors Microbiology, March 1, 2004; 150(3): 591 - 599. [Abstract] [Full Text] [PDF]

				C. K. Cote and A. L. Honeyman The LicT protein acts as both a positive and a negative regulator of loci within the bgl regulon of Streptococcus mutans Microbiology, May 1, 2003; 149(5): 1333 - 1340. [Abstract] [Full Text] [PDF]

				M. Gobel, K. Kassel-Cati, E. Schmidt, and W. Reineke Degradation of Aromatics and Chloroaromatics by Pseudomonas sp. Strain B13: Cloning, Characterization, and Analysis of Sequences Encoding 3-Oxoadipate:Succinyl-Coenzyme A (CoA) Transferase and 3-Oxoadipyl-CoA Thiolase J. Bacteriol., January 1, 2002; 184(1): 216 - 223. [Abstract] [Full Text] [PDF]

				R. H. Ffrench-Constant, N. Waterfield, V. Burland, N. T. Perna, P. J. Daborn, D. Bowen, and F. R. Blattner A Genomic Sample Sequence of the Entomopathogenic Bacterium Photorhabdus luminescens W14: Potential Implications for Virulence Appl. Envir. Microbiol., August 1, 2000; 66(8): 3310 - 3329. [Abstract] [Full Text]

				K.-i. Yoshida, I. Ishio, E. Nagakawa, Y. Yamamoto, M. Yamamoto, and Y. Fujita Systematic study of gene expression and transcription organization in the gntZ-ywaA region of the Bacillus subtilis genome Microbiology, March 1, 2000; 146(3): 573 - 579. [Abstract] [Full Text]

				K. Kossen and O. C. Uhlenbeck Cloning and biochemical characterization of Bacillus subtilis YxiN, a DEAD protein specifically activated by 23S rRNA: delineation of a novel sub-family of bacterial DEAD proteins Nucleic Acids Res., October 1, 1999; 27(19): 3811 - 3820. [Abstract] [Full Text] [PDF]

				G. Greller, R. Horlacher, J. DiRuggiero, and W. Boos Molecular and Biochemical Analysis of MalK, the ATP-hydrolyzing Subunit of the Trehalose/Maltose Transport System of the Hyperthermophilic Archaeon Thermococcus litoralis J. Biol. Chem., July 16, 1999; 274(29): 20259 - 20264. [Abstract] [Full Text] [PDF]

				J. Armengaud, K. N. Timmis, and R.-M. Wittich A Functional 4-Hydroxysalicylate/Hydroxyquinol Degradative Pathway Gene Cluster Is Linked to the Initial Dibenzo-p-Dioxin Pathway Genes in Sphingomonas sp. Strain RW1 J. Bacteriol., June 1, 1999; 181(11): 3452 - 3461. [Abstract] [Full Text]

				L. Winstedt, K.-I. Yoshida, Y. Fujita, and C. von Wachenfeldt Cytochrome bd Biosynthesis in Bacillus subtilis: Characterization of the cydABCD Operon J. Bacteriol., December 15, 1998; 180(24): 6571 - 6580. [Abstract] [Full Text]

				Y. Fujita, K.-I. Yoshida, Y. Miwa, N. Yanai, E. Nagakawa, and Y. Kasahara Identification and Expression of the Bacillus subtilis Fructose-1,6-Bisphosphatase Gene (fbp) J. Bacteriol., August 15, 1998; 180(16): 4309 - 4313. [Abstract] [Full Text]

				I. Bagyan, L. Casillas-Martinez, and P. Setlow The katX Gene, Which Codes for the Catalase in Spores of Bacillus subtilis, Is a Forespore-Specific Gene Controlled by sigma F, and KatX Is Essential for Hydrogen Peroxide Resistance of the Germinating Spore J. Bacteriol., April 15, 1998; 180(8): 2057 - 2062. [Abstract] [Full Text]