| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
microbiology, Vol 142, 3437-3444, Copyright © 1996 by Society for General Microbiology
ARTICLES |
P Margot and D Karamata
Institut de genetique et de biologie microbiennes, Lausanne, Switzerland.
The nucleotide sequence of wprA, a protease-encoding gene of Bacillus subtilis 168, is reported. The gene, expressed during the exponential growth phase, belongs to a monocistronic operon. WprA is a 96 kDa polypeptide endowed with a signal peptide, as well as a propeptide. Upon processing and export, it gives rise to two previously identified cell-wall-bound proteins, CWBP23 and 52. Processing of WprA exhibits a novel feature of protein export, whereby removal of the middle part of the molecule accompanies the targeting to the cell wall of its N- and C- terminal parts, which correspond to CWBP23 and 52, respectively. Sequence analyses and enzymic assays reveal that CWBP52 is a serine protease. Growth rate, cell morphology, sporulation and motility of wprA mutants apparently do not differ from those of the parent strain.
This article has been cited by other articles:
|
|
 |
|
  C. Baptista, M. A. Santos, and C. Sao-Jose Phage SPP1 Reversible Adsorption to Bacillus subtilis Cell Wall Teichoic Acids Accelerates Virus Recognition of Membrane Receptor YueB J. Bacteriol., July 15, 2008; 190(14): 4989 - 4996. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. J. B. Sibbald, A. K. Ziebandt, S. Engelmann, M. Hecker, A. de Jong, H. J. M. Harmsen, G. C. Raangs, I. Stokroos, J. P. Arends, J. Y. F. Dubois, et al. Mapping the Pathways to Staphylococcal Pathogenesis by Comparative Secretomics Microbiol. Mol. Biol. Rev., September 1, 2006; 70(3): 755 - 788. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Sao-Jose, C. Baptista, and M. A. Santos Bacillus subtilis Operon Encoding a Membrane Receptor for Bacteriophage SPP1 J. Bacteriol., December 15, 2004; 186(24): 8337 - 8346. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. H. Park, S. J. Lee, S. G. Lee, W. S. Lee, and S. M. Byun Hetero- and Autoprocessing of the Extracellular Metalloprotease (Mpr) in Bacillus subtilis J. Bacteriol., October 1, 2004; 186(19): 6457 - 6464. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bindel Connelly, G. M. Young, and A. Sloma Extracellular Proteolytic Activity Plays a Central Role in Swarming Motility in Bacillus subtilis J. Bacteriol., July 1, 2004; 186(13): 4159 - 4167. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Tjalsma, H. Antelmann, J. D.H. Jongbloed, P. G. Braun, E. Darmon, R. Dorenbos, J.-Y. F. Dubois, H. Westers, G. Zanen, W. J. Quax, et al. Proteomics of Protein Secretion by Bacillus subtilis: Separating the "Secrets" of the Secretome Microbiol. Mol. Biol. Rev., June 1, 2004; 68(2): 207 - 233. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Grass, A. Schierhorn, E. Sorkau, H. Muller, P. Rucknagel, D. H. Nies, and B. Fricke Camelysin Is a Novel Surface Metalloproteinase from Bacillus cereus Infect. Immun., January 1, 2004; 72(1): 219 - 228. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Yamamoto, S.-i. Kurosawa, and J. Sekiguchi Localization of the Vegetative Cell Wall Hydrolases LytC, LytE, and LytF on the Bacillus subtilis Cell Surface and Stability of These Enzymes to Cell Wall-Bound or Extracellular Proteases J. Bacteriol., November 15, 2003; 185(22): 6666 - 6677. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Lian, S. J. Szarka, K. K. S. Ng, and S.-L. Wong Engineering of a Staphylokinase-based Fibrinolytic Agent with Antithrombotic Activity and Targeting Capability toward Thrombin-rich Fibrin and Plasma Clots J. Biol. Chem., July 11, 2003; 278(29): 26677 - 26686. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-E. Germond, M. Delley, C. Gilbert, and D. Atlan Determination of the Domain of the Lactobacillus delbrueckii subsp. bulgaricus Cell Surface Proteinase PrtB Involved in Attachment to the Cell Wall after Heterologous Expression of the prtB Gene in Lactococcus lactis Appl. Envir. Microbiol., June 1, 2003; 69(6): 3377 - 3384. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Wahlstrom, M. Vitikainen, V. P. Kontinen, and M. Sarvas The extracytoplasmic folding factor PrsA is required for protein secretion only in the presence of the cell wall in Bacillus subtilis Microbiology, March 1, 2003; 149(3): 569 - 577. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-C. Wu, J. C. Yeung, Y. Duan, R. Ye, S. J. Szarka, H. R. Habibi, and S.-L. Wong Functional Production and Characterization of a Fibrin-Specific Single-Chain Antibody Fragment from Bacillus subtilis: Effects of Molecular Chaperones and a Wall-Bound Protease on Antibody Fragment Production Appl. Envir. Microbiol., July 1, 2002; 68(7): 3261 - 3269. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Miyoshi, I. Poquet, V. Azevedo, J. Commissaire, L. Bermudez-Humaran, E. Domakova, Y. Le Loir, S. C. Oliveira, A. Gruss, and P. Langella Controlled Production of Stable Heterologous Proteins in Lactococcus lactis Appl. Envir. Microbiol., June 1, 2002; 68(6): 3141 - 3146. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Murashima, C.-L. Chen, A. Kosugi, Y. Tamaru, R. H. Doi, and S.-L. Wong Heterologous Production of Clostridium cellulovorans engB, Using Protease-Deficient Bacillus subtilis, and Preparation of Active Recombinant Cellulosomes J. Bacteriol., January 1, 2002; 184(1): 76 - 81. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Thwaite, L. W. J. Baillie, N. M. Carter, K. Stephenson, M. Rees, C. R. Harwood, and P. T. Emmerson Optimization of the Cell Wall Microenvironment Allows Increased Production of Recombinant Bacillus anthracis Protective Antigen from B. subtilis Appl. Envir. Microbiol., January 1, 2002; 68(1): 227 - 234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Antelmann, H. Tjalsma, B. Voigt, S. Ohlmeier, S. Bron, J. M. van Dijl, and M. Hecker A Proteomic View on Genome-Based Signal Peptide Predictions Genome Res., September 1, 2001; 11(9): 1484 - 1502. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Tjalsma, A. Bolhuis, J. D. H. Jongbloed, S. Bron, and J. M. van Dijl Signal Peptide-Dependent Protein Transport in Bacillus subtilis: a Genome-Based Survey of the Secretome Microbiol. Mol. Biol. Rev., September 1, 2000; 64(3): 515 - 547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Lee, D. M. Kim, K. H. Bae, S. M. Byun, and J. H. Chung Enhancement of Secretion and Extracellular Stability of Staphylokinase in Bacillus subtilis by wprA Gene Disruption Appl. Envir. Microbiol., February 1, 2000; 66(2): 476 - 480. [Abstract] [Full Text]
|
|
|
|
|
|
I. Hirose, K. Sano, I. Shioda, M. Kumano, K. Nakamura, and K. Yamane Proteome analysis of Bacillus subtilis extracellular proteins: a two-dimensional protein electrophoretic study Microbiology, January 1, 2000; 146(1): 65 - 75. [Abstract] [Full Text]
|
|
|
|
|
|
A. Bolhuis, H. Tjalsma, H. E. Smith, A. de Jong, R. Meima, G. Venema, S. Bron, and J. M. van Dijl Evaluation of Bottlenecks in the Late Stages of Protein Secretion in Bacillus subtilis Appl. Envir. Microbiol., July 1, 1999; 65(7): 2934 - 2941. [Abstract] [Full Text]
|
|
|
|
|
|
A. Bolhuis, H. Tjalsma, K. Stephenson, C. R. Harwood, G. Venema, S. Bron, and J. M. van Dijl Different Mechanisms for Thermal Inactivation of Bacillus subtilis Signal Peptidase Mutants J. Biol. Chem., May 28, 1999; 274(22): 15865 - 15868. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Stephenson and C. R. Harwood Influence of a Cell-Wall-Associated Protease on Production of alpha -Amylase by Bacillus subtilis Appl. Envir. Microbiol., August 1, 1998; 64(8): 2875 - 2881. [Abstract] [Full Text]
|
|
|
|
|
|
S.-C. Wu, R. Ye, X.-C. Wu, S.-C. Ng, and S.-L. Wong Enhanced Secretory Production of a Single-Chain Antibody Fragment from Bacillus subtilis by Coproduction of Molecular Chaperones J. Bacteriol., June 1, 1998; 180(11): 2830 - 2835. [Abstract] [Full Text]
|
|
|
|
|
|
P. Margot, M. Wahlen, A. Gholamhuseinian, P. Piggot, and D. Karamata The lytE Gene of Bacillus subtilis 168 Encodes a Cell Wall Hydrolase J. Bacteriol., February 1, 1998; 180(3): 749 - 752. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
