Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Microbiology 142 (1996), 401-410
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tabata, K.
Right arrow Articles by Hoshino, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tabata, K.
Right arrow Articles by Hoshino, T.
Agricola
Right arrow Articles by Tabata, K.
Right arrow Articles by Hoshino, T.

microbiology, Vol 142, 401-410, Copyright © 1996 by Society for General Microbiology

ARTICLES

Mapping of 61 genes on the refined physical map of the chromosome of Thermus thermophilus HB27 and comparison of genome organization with that of T. thermophilus HB8

K Tabata and T Hoshino
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.

We have constructed refined physical maps of the chromosome (1 center dot 82 Mb) and the large plasmid pTT27 (250 kb) of Thermus thermophilus HB27. A total of 49 cleavage sites with five restriction enzymes, EcoRI, SspI, MunI, EcoRV and ClaI, were determined on the maps. The location of 61 genes was determined by using as probes 64 genes cloned from T. thermophilus or other Thermus strains. Comparison of the genomic organization of the chromosomes of T. thermophilus HB27 and HB8 revealed that they were basically identical, but some genes were located in different regions. Among 32 genes whose locations were determined on both the HB27 and the HB8 chromosomes, the copy number of rpsL-rpsG-fus-tufA, the locations of glyS, pol, and one copy of nusG- rplK-rplA were different. The IS1000 sequence was located only in one region on the HB27 chromosome. In contrast, IS1000 sequences were scattered over four regions on the chromosome of HB8. As each region in which glyS, pol, or one copy of nusG-rplK-rplA are present also contained IS1000 in HB8, it is suggested that IS1000 may play an important role in genomic rearrangements in Thermus strains.


This article has been cited by other articles:


Home page
 J. Bacteriol.Home page
P. Le Bourgeois, M.-L. Daveran-Mingot, and P. Ritzenthaler
Genome Plasticity among Related Lactococcus Strains: Identification of Genetic Events Associated with Macrorestriction Polymorphisms
J. Bacteriol., May 1, 2000; 182(9): 2481 - 2491.
[Abstract] [Full Text]

Home page
 Appl. Environ. Microbiol.Home page
T. Kosuge and T. Hoshino
Construction of a Proline-Producing Mutant of the Extremely Thermophilic Eubacterium Thermus thermophilus HB27
Appl. Envir. Microbiol., November 1, 1998; 64(11): 4328 - 4332.
[Abstract] [Full Text]

Home page
 J. Bacteriol.Home page
M.-L. Daveran-Mingot, N. Campo, P. Ritzenthaler, and P. Le Bourgeois
A Natural Large Chromosomal Inversion in Lactococcus lactis Is Mediated by Homologous Recombination between Two Insertion Sequences
J. Bacteriol., September 15, 1998; 180(18): 4834 - 4842.
[Abstract] [Full Text]

Home page
 MicrobiologyHome page
M Baetens, C Legrain, A Boyen, and N Glansdorff
Genes and enzymes of the acetyl cycle of arginine biosynthesis in the extreme thermophilic bacterium Thermus thermophilus HB27
Microbiology, February 1, 1998; 144(2): 479 - 492.
[Abstract]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1996 Society for General Microbiology.