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Lehrstuhl für Mikrobiologie, Technische Universität München, D-80290 München, Germany
Laboratorium voor Microbiologie, Universiteit Gent, Belgium
3Author for correspondence: Werner Manz. Tel: +49 30 314 25831. Fax: +49 30 314 73461. e-mail: manz0654@mailszrz.zrz.tu-berlin.de
ABSTRACT
We designed a panel of four 16S rRNA-targeted oligonucleotide probes specific for bacteria of the phylum cytophaga-flavobacter-bacteroides (CFB). Probes CF319a and CF319b are targeted to members of the flavobacteria-cytophaga group and the genus Porphyromonas, whereas probe BAC303 has a target region characteristic for the genera Prevotella and Bacteroides within the bacteroides group. The probe FFE8b was developed for species-specific hybridizations with Flavobacterium ferrugineum. All probes were designed by computer-assisted sequence analysis and compared to all currently accessible 16S and 23S rRNA sequences. The oligonucleotides were further evaluated by whole-cell and non-radioactive dot-blot hybridization against reference strains of the CFB phylum and other major lineages of Bacteria. The newly developed probes were used together with other higher-order probes to analyse the structure and community composition in complex environments. In activated sludge samples, members of the flavobacteria-cytophaga group were revealed by in situ hybridization as important constituents of sludge flocs and characteristic colonizers of filamentous bacteria. By application of fluorescent probe BAC303, members of the genera Bacteroides and Prevotella could be visualized without prior cultivation as an important part of the human faecal microflora.
Present address: Fachgebiet ökologie der Mikroorganismen, Technische Universität Berlin, D-10587 Berlin, Germany.
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R. Sekar, B. M. Fuchs, R. Amann, and J. Pernthaler Flow Sorting of Marine Bacterioplankton after Fluorescence In Situ Hybridization Appl. Envir. Microbiol., October 1, 2004; 70(10): 6210 - 6219. [Abstract] [Full Text] [PDF] |
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J. Pernthaler, E. Zollner, F. Warnecke, and K. Jurgens Bloom of Filamentous Bacteria in a Mesotrophic Lake: Identity and Potential Controlling Mechanism Appl. Envir. Microbiol., October 1, 2004; 70(10): 6272 - 6281. [Abstract] [Full Text] [PDF] |
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C.-L. Chen, H. Macarie, I. Ramirez, A. Olmos, S. L. Ong, O. Monroy, and W.-T. Liu Microbial community structure in a thermophilic anaerobic hybrid reactor degrading terephthalate Microbiology, October 1, 2004; 150(10): 3429 - 3440. [Abstract] [Full Text] [PDF] |
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J. Vulevic, A. L. McCartney, J. M. Gee, I. T. Johnson, and G. R. Gibson Microbial Species Involved in Production of 1,2-sn-Diacylglycerol and Effects of Phosphatidylcholine on Human Fecal Microbiota Appl. Envir. Microbiol., September 1, 2004; 70(9): 5659 - 5666. [Abstract] [Full Text] [PDF] |
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H. M. Probert, J. H. A. Apajalahti, N. Rautonen, J. Stowell, and G. R. Gibson Polydextrose, Lactitol, and Fructo-Oligosaccharide Fermentation by Colonic Bacteria in a Three-Stage Continuous Culture System Appl. Envir. Microbiol., August 1, 2004; 70(8): 4505 - 4511. [Abstract] [Full Text] [PDF] |
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U. Stingl, A. Maass, R. Radek, and A. Brune Symbionts of the gut flagellate Staurojoenina sp. from Neotermes cubanus represent a novel, termite-associated lineage of Bacteroidales: description of 'Candidatus Vestibaculum illigatum' Microbiology, July 1, 2004; 150(7): 2229 - 2235. [Abstract] [Full Text] [PDF] |
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M. Schuppler, K. Lotzsch, M. Waidmann, and I. B. Autenrieth An Abundance of Escherichia coli Is Harbored by the Mucosa- Associated Bacterial Flora of Interleukin-2-Deficient Mice Infect. Immun., April 1, 2004; 72(4): 1983 - 1990. [Abstract] [Full Text] [PDF] |
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A Fite, G T Macfarlane, J H Cummings, M J Hopkins, S C Kong, E Furrie, and S Macfarlane Identification and quantitation of mucosal and faecal desulfovibrios using real time polymerase chain reaction Gut, April 1, 2004; 53(4): 523 - 529. [Abstract] [Full Text] [PDF] |
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T. Kindaichi, T. Ito, and S. Okabe Ecophysiological Interaction between Nitrifying Bacteria and Heterotrophic Bacteria in Autotrophic Nitrifying Biofilms as Determined by Microautoradiography-Fluorescence In Situ Hybridization Appl. Envir. Microbiol., March 1, 2004; 70(3): 1641 - 1650. [Abstract] [Full Text] [PDF] |
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M. Sunamura, Y. Higashi, C. Miyako, J.-i. Ishibashi, and A. Maruyama Two Bacteria Phylotypes Are Predominant in the Suiyo Seamount Hydrothermal Plume Appl. Envir. Microbiol., February 1, 2004; 70(2): 1190 - 1198. [Abstract] [Full Text] [PDF] |
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V. Mai, H. A. Katki, H. Harmsen, D. Gallaher, A. Schatzkin, D. J. Baer, and B. Clevidence Effects of a Controlled Diet and Black Tea Drinking on the Fecal Microflora Composition and the Fecal Bile Acid Profile of Human Volunteers in a Double-Blinded Randomized Feeding Study J. Nutr., February 1, 2004; 134(2): 473 - 478. [Abstract] [Full Text] [PDF] |
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F Hoentjen, H J M Harmsen, H Braat, C D Torrice, B A Mann, R B Sartor, and L A Dieleman Antibiotics with a selective aerobic or anaerobic spectrum have different therapeutic activities in various regions of the colon in interleukin 10 gene deficient mice Gut, December 1, 2003; 52(12): 1721 - 1727. [Abstract] [Full Text] [PDF] |
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R. Brinkmeyer, K. Knittel, J. Jurgens, H. Weyland, R. Amann, and E. Helmke Diversity and Structure of Bacterial Communities in Arctic versus Antarctic Pack Ice Appl. Envir. Microbiol., November 1, 2003; 69(11): 6610 - 6619. [Abstract] [Full Text] [PDF] |
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D. Schmitt-Wagner, M. W. Friedrich, B. Wagner, and A. Brune Phylogenetic Diversity, Abundance, and Axial Distribution of Bacteria in the Intestinal Tract of Two Soil-Feeding Termites (Cubitermes spp.) Appl. Envir. Microbiol., October 1, 2003; 69(10): 6007 - 6017. [Abstract] [Full Text] [PDF] |
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A. S. Engel, N. Lee, M. L. Porter, L. A. Stern, P. C. Bennett, and M. Wagner Filamentous "Epsilonproteobacteria" Dominate Microbial Mats from Sulfidic Cave Springs Appl. Envir. Microbiol., September 1, 2003; 69(9): 5503 - 5511. [Abstract] [Full Text] [PDF] |
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J. Bertaux, M. Schmid, N. C. Prevost-Boure, J. L. Churin, A. Hartmann, J. Garbaye, and P. Frey-Klett In Situ Identification of Intracellular Bacteria Related to Paenibacillus spp. in the Mycelium of the Ectomycorrhizal Fungus Laccaria bicolor S238N Appl. Envir. Microbiol., July 1, 2003; 69(7): 4243 - 4248. [Abstract] [Full Text] [PDF] |
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J. Peplies, F. O. Glockner, and R. Amann Optimization Strategies for DNA Microarray-Based Detection of Bacteria with 16S rRNA-Targeting Oligonucleotide Probes Appl. Envir. Microbiol., March 1, 2003; 69(3): 1397 - 1407. [Abstract] [Full Text] [PDF] |
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S. H. Duncan, K. P. Scott, A. G. Ramsay, H. J. M. Harmsen, G. W. Welling, C. S. Stewart, and H. J. Flint Effects of Alternative Dietary Substrates on Competition between Human Colonic Bacteria in an Anaerobic Fermentor System Appl. Envir. Microbiol., February 1, 2003; 69(2): 1136 - 1142. [Abstract] [Full Text] [PDF] |
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G. R. Crocetti, J. F. Banfield, J. Keller, P. L. Bond, and L. L. Blackall Glycogen-accumulating organisms in laboratory-scale and full-scale wastewater treatment processes Microbiology, November 1, 2002; 148(11): 3353 - 3364. [Abstract] [Full Text] [PDF] |
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N. H. Salzman, H. de Jong, Y. Paterson, H. J. M. Harmsen, G. W. Welling, and N. A. Bos Analysis of 16S libraries of mouse gastrointestinal microflora reveals a large new group of mouse intestinal bacteria Microbiology, November 1, 2002; 148(11): 3651 - 3660. [Abstract] [Full Text] [PDF] |
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J.-P. Gattuso, S. Peduzzi, M.-D. Pizay, and M. Tonolla Changes in freshwater bacterial community composition during measurements of microbial and community respiration J. Plankton Res., November 1, 2002; 24(11): 1197 - 1206. [Abstract] [Full Text] [PDF] |
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K. E. Ashelford, A. J. Weightman, and J. C. Fry PRIMROSE: a computer program for generating and estimating the phylogenetic range of 16S rRNA oligonucleotide probes and primers in conjunction with the RDP-II database Nucleic Acids Res., August 1, 2002; 30(15): 3481 - 3489. [Abstract] [Full Text] [PDF] |
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P V Kirjavainen, T Arvola, S J Salminen, and E Isolauri Aberrant composition of gut microbiota of allergic infants: a target of bifidobacterial therapy at weaning? Gut, July 1, 2002; 51(1): 51 - 55. [Abstract] [Full Text] [PDF] |
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H. J. M. Harmsen, G. C. Raangs, T. He, J. E. Degener, and G. W. Welling Extensive Set of 16S rRNA-Based Probes for Detection of Bacteria in Human Feces Appl. Envir. Microbiol., June 1, 2002; 68(6): 2982 - 2990. [Abstract] [Full Text] [PDF] |
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E. O. Casamayor, C. Pedros-Alio, G. Muyzer, and R. Amann Microheterogeneity in 16S Ribosomal DNA-Defined Bacterial Populations from a Stratified Planktonic Environment Is Related to Temporal Changes and to Ecological Adaptations Appl. Envir. Microbiol., April 1, 2002; 68(4): 1706 - 1714. [Abstract] [Full Text] [PDF] |
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