| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Biochemistry, Arrhenius Laboratories for Natural Sciences, Stockholm University, S-10691 Stockholm, Sweden
2Author for correspondence: Stefan Nordlund. Tel: +46 8 16 29 32. Fax: +46 8 15 77 94. email: stefan@biokemi.su.se
ABSTRACT
The P11 protein, encoded by glnB, has a central role in the control of nitrogen metabolism in nitrogen-fixing prokaryotes. The glnB gene of Rhodospirillum rubrum was isolated and sequenced. The deduced amino acid sequence had very high sequence identity to other P11 proteins. The glnA gene, encoding glutamine synthetase, was located 135 bp downstream of glnB and was partially sequenced. glnB is cotranscribed with glnA from a promoter with high similarity to the s54-dependent promoter consensus sequence. A putative s70 promoter was also identified further upstream of glnB. Northern blotting analyses showed that in addition glnA is either transcribed from an unidentified promoter or, more likely, that the glnBA transcript is processed to give the glnA mRNA. The total level of the two transcripts was much higher in nitrogen-fixing cells than in ammonia-grown cells.
This article has been cited by other articles:
|
|
 |
|
  P. F. Teixeira, A. Jonsson, M. Frank, H. Wang, and S. Nordlund Interaction of the signal transduction protein GlnJ with the cellular targets AmtB1, GlnE and GlnD in Rhodospirillum rubrum: dependence on manganese, 2-oxoglutarate and the ADP/ATP ratio Microbiology, August 1, 2008; 154(8): 2336 - 2347. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zhang, E. L. Pohlmann, and G. P. Roberts GlnD Is Essential for NifA Activation, NtrB/NtrC-Regulated Gene Expression, and Posttranslational Regulation of Nitrogenase Activity in the Photosynthetic, Nitrogen-Fixing Bacterium Rhodospirillum rubrum J. Bacteriol., February 15, 2005; 187(4): 1254 - 1265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Edgren and S. Nordlund The fixABCX Genes in Rhodospirillum rubrum Encode a Putative Membrane Complex Participating in Electron Transfer to Nitrogenase J. Bacteriol., April 1, 2004; 186(7): 2052 - 2060. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Perlova, A. Ureta, S. Nordlund, and D. Meletzus Identification of Three Genes Encoding PII-Like Proteins in Gluconacetobacter diazotrophicus: Studies of Their Role(s) in the Control of Nitrogen Fixation J. Bacteriol., October 1, 2003; 185(19): 5854 - 5861. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Arcondeguy, R. Jack, and M. Merrick PII Signal Transduction Proteins, Pivotal Players in Microbial Nitrogen Control Microbiol. Mol. Biol. Rev., March 1, 2001; 65(1): 80 - 105. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhang, E. L. Pohlmann, P. W. Ludden, and G. P. Roberts Mutagenesis and Functional Characterization of the glnB, glnA, and nifA Genes from the Photosynthetic Bacterium Rhodospirillum rubrum J. Bacteriol., February 15, 2000; 182(4): 983 - 992. [Abstract] [Full Text]
|
|
|
|
|
|
M. Johansson and S. Nordlund Purification of PII and PII-UMP and In Vitro Studies of Regulation of Glutamine Synthetase in Rhodospirillum rubrum J. Bacteriol., October 15, 1999; 181(20): 6524 - 6529. [Abstract] [Full Text]
|
|
|
|
|
|
J. Cheng, M. Johansson, and S. Nordlund Expression of PII and Glutamine Synthetase Is Regulated by PII, the ntrBC Products, and Processing of the glnBA mRNA in Rhodospirillum rubrum J. Bacteriol., October 15, 1999; 181(20): 6530 - 6534. [Abstract] [Full Text]
|
|
|
|
|
|
H.-M. Lee, M. F. Vázquez-Bermúdez, and N. T. de Marsac The Global Nitrogen Regulator NtcA Regulates Transcription of the Signal Transducer PII (GlnB) and Influences Its Phosphorylation Level in Response to Nitrogen and Carbon Supplies in the Cyanobacterium Synechococcus sp. Strain PCC 7942 J. Bacteriol., May 1, 1999; 181(9): 2697 - 2702. [Abstract] [Full Text]
|
|
|
|
|
|
R. Jack, M. De Zamaroczy, and M. Merrick The Signal Transduction Protein GlnK Is Required for NifL-Dependent Nitrogen Control of nif Gene Expression in Klebsiella pneumoniae J. Bacteriol., February 15, 1999; 181(4): 1156 - 1162. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Qian and F. R. Tabita Expression of glnB and a glnB-Like Gene (glnK) in a Ribulose Bisphosphate Carboxylase/Oxygenase-Deficient Mutant of Rhodobacter sphaeroides J. Bacteriol., September 1, 1998; 180(17): 4644 - 4649. [Abstract] [Full Text]
|
|
|
|
|
|
D. Meletzus, P. Rudnick, N. Doetsch, A. Green, and C. Kennedy J. Bacteriol., June 15, 1998; 180(12): 3260 - 3264. [Abstract]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
