| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Institut für Biotechnologie 1, Forschungszentrum Jülich, D-52425 Jülich, Germany
2Author for correspondence: Bernhard J. Eikmanns. Tel: +49 2461 61 3967. Fax: +49 2461 61 2710.
ABSTRACT
Relatively limited information about promoter structures in Corynebacterium glutamicum has been available until now. With the aim of isolating and characterizing such transcription initiation signals, random Sau3A fragments of C. glutamicum chromosomal DNA and of the corynebacterial phage øGA1 were cloned into the promoter probe vector pEKplCm and selected for promoter activity by chloramphenicol resistance of transformed C. glutamicum cells. The nucleotide sequence of ten chromosomal and three phage fragments was determined and the transcriptional start (TS) sites were localized by primer extension analyses. Additionally, the promoters of five previously isolated C. glutamicum genes were cloned and mapped. All of the isolated promoters were also functional in the heterologous host Escherichia coli. A comparative analysis of the newly characterized promoter sequences together with published promoters from C. glutamicum revealed conserved sequences centred about 35 bp (ttGcca) and 10 bp (TA.aaT) upstream of the TS site. The position of these motifs and the motifs themselves are comparable to the –35 and –10 promoter consensus sequences of other Gram-positive and Gram-negative bacteria, indicating that they represent transcription initiation signals in C. glutamicum. However, the C. glutamicum consensus hexamer of the –35 region is much less conserved than in E. coli, Bacillus, Lactobacillus and Streptococcus.
Present address: Institute of Thermomechanics, Academy of Sciences of the Czech Republic, Dolejskova 5, CZ-18200 Praha 8, Czech Republic.
Present address: Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnská 1083, CZ-14220 Praha 4, Czech Republic.
This article has been cited by other articles:
|
|
 |
|
  A. Arndt and B. J. Eikmanns The Alcohol Dehydrogenase Gene adhA in Corynebacterium glutamicum Is Subject to Carbon Catabolite Repression J. Bacteriol., October 15, 2007; 189(20): 7408 - 7416. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chen, M. Bagdasarian, M. G. Kaufman, A. K. Bates, and E. D. Walker Mutational Analysis of the ompA Promoter from Flavobacterium johnsoniae J. Bacteriol., July 15, 2007; 189(14): 5108 - 5118. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Asakura, E. Kimura, Y. Usuda, Y. Kawahara, K. Matsui, T. Osumi, and T. Nakamatsu Altered Metabolic Flux due to Deletion of odhA causes L-Glutamate Overproduction in Corynebacterium glutamicum Appl. Envir. Microbiol., February 15, 2007; 73(4): 1308 - 1319. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Cramer, M. Auchter, J. Frunzke, M. Bott, and B. J. Eikmanns RamB, the Transcriptional Regulator of Acetate Metabolism in Corynebacterium glutamicum, Is Subject to Regulation by RamA and RamB J. Bacteriol., February 1, 2007; 189(3): 1145 - 1149. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Hansmeier, A. Albersmeier, A. Tauch, T. Damberg, R. Ros, D. Anselmetti, A. Puhler, and J. Kalinowski The surface (S)-layer gene cspB of Corynebacterium glutamicum is transcriptionally activated by a LuxR-type regulator and located on a 6 kb genomic island absent from the type strain ATCC 13032. Microbiology, April 1, 2006; 152(Pt 4): 923 - 935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Schreiner, C. Riedel, J. Holatko, M. Patek, and B. J. Eikmanns Pyruvate:Quinone Oxidoreductase in Corynebacterium glutamicum: Molecular Analysis of the pqo Gene, Significance of the Enzyme, and Phylogenetic Aspects J. Bacteriol., February 15, 2006; 188(4): 1341 - 1350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Letek, N. Valbuena, A. Ramos, E. Ordonez, J. A. Gil, and L. M. Mateos Characterization and Use of Catabolite-Repressed Promoters from Gluconate Genes in Corynebacterium glutamicum J. Bacteriol., January 15, 2006; 188(2): 409 - 423. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Barriuso-Iglesias, C. Barreiro, F. Flechoso, and J. F. Martin Transcriptional analysis of the F0F1 ATPase operon of Corynebacterium glutamicum ATCC 13032 reveals strong induction by alkaline pH Microbiology, January 1, 2006; 152(1): 11 - 21. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Schreiner, D. Fiur, J. Holatko, M. Patek, and B. J. Eikmanns E1 Enzyme of the Pyruvate Dehydrogenase Complex in Corynebacterium glutamicum: Molecular Analysis of the Gene and Phylogenetic Aspects J. Bacteriol., September 1, 2005; 187(17): 6005 - 6018. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Rittmann, U. Sorger-Herrmann, and V. F. Wendisch Phosphate Starvation-Inducible Gene ushA Encodes a 5' Nucleotidase Required for Growth of Corynebacterium glutamicum on Media with Nucleotides as the Phosphorus Source Appl. Envir. Microbiol., August 1, 2005; 71(8): 4339 - 4344. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Barreiro, E. Gonzalez-Lavado, M. Patek, and J.-F. Martin Transcriptional Analysis of the groES-groEL1, groEL2, and dnaK genes in Corynebacterium glutamicum: Characterization of Heat Shock-Induced Promoters J. Bacteriol., July 15, 2004; 186(14): 4813 - 4817. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ramos, M. P. Honrubia, N. Valbuena, J. Vaquera, L. M. Mateos, and J. A. Gil Involvement of DivIVA in the morphology of the rod-shaped actinomycete Brevibacterium lactofermentum Microbiology, December 1, 2003; 149(12): 3531 - 3542. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Venkova-Canova, M. Patek, and J. Nesvera Control of rep Gene Expression in Plasmid pGA1 from Corynebacteriumglutamicum J. Bacteriol., April 15, 2003; 185(8): 2402 - 2409. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. I. Trindade, V. R. Abratt, and S. J. Reid Induction of Sucrose Utilization Genes from Bifidobacterium lactis by Sucrose and Raffinose Appl. Envir. Microbiol., January 1, 2003; 69(1): 24 - 32. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Kennerknecht, H. Sahm, M.-R. Yen, M. Patek, M. H. Saier Jr., and L. Eggeling Export of L-Isoleucine from Corynebacterium glutamicum: a Two-Gene-Encoded Member of a New Translocator Family J. Bacteriol., July 15, 2002; 184(14): 3947 - 3956. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Simic, J. Willuhn, H. Sahm, and L. Eggeling Identification of glyA (Encoding Serine Hydroxymethyltransferase) and Its Use Together with the Exporter ThrE To Increase L-Threonine Accumulation by Corynebacterium glutamicum Appl. Envir. Microbiol., July 1, 2002; 68(7): 3321 - 3327. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Beckers, L. Nolden, and A. Burkovski Glutamate synthase of Corynebacterium glutamicum is not essential for glutamate synthesis and is regulated by the nitrogen status Microbiology, November 1, 2001; 147(11): 2961 - 2970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Simic, H. Sahm, and L. Eggeling L-Threonine Export: Use of Peptides To Identify a New Translocator from Corynebacterium glutamicum J. Bacteriol., September 15, 2001; 183(18): 5317 - 5324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Schwinde, P. F. Hertz, H. Sahm, B. J. Eikmanns, and A. Guyonvarch Lipoamide dehydrogenase from Corynebacterium glutamicum: molecular and physiological analysis of the lpd gene and characterization of the enzyme Microbiology, August 1, 2001; 147(8): 2223 - 2231. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Barreiro, E. González-Lavado, and J. F. Martín Organization and Transcriptional Analysis of a Six-Gene Cluster around the rplK-rplA Operon of Corynebacterium glutamicum Encoding the Ribosomal Proteins L11 and L1 Appl. Envir. Microbiol., May 1, 2001; 67(5): 2183 - 2190. [Abstract] [Full Text]
|
|
|
|
|
|
S. M. Doree and M. H. Mulks Identification of an Actinobacillus pleuropneumoniae Consensus Promoter Structure J. Bacteriol., March 15, 2001; 183(6): 1983 - 1989. [Abstract] [Full Text]
|
|
|
|
|
|
L. Wehmeier, O. Brockmann-Gretza, A. Pisabarro, A. Tauch, A. Pühler, J. F. Martin, and J. Kalinowski A Corynebacterium glutamicum mutant with a defined deletion within the rplK gene is impaired in (p)ppGpp accumulation upon amino acid starvation Microbiology, March 1, 2001; 147(3): 691 - 700. [Abstract] [Full Text]
|
|
|
|
|
|
M. Merkamm and A. Guyonvarch Cloning of the sodA Gene from Corynebacterium melassecola and Role of Superoxide Dismutase in Cellular Viability J. Bacteriol., February 15, 2001; 183(4): 1284 - 1295. [Abstract] [Full Text]
|
|
|
|
|
|
M. Pátek, H. Sahm, and B. Eikmanns Analysis of the Corynebacterium glutamicum dapA Promoter J. Bacteriol., October 1, 1999; 181(19): 6188 - 6191. [Abstract] [Full Text]
|
|
|
|
|
|
N. Dusch, A. Pühler, and J. Kalinowski Expression of the Corynebacterium glutamicum panD Gene Encoding L-Aspartate-alpha -Decarboxylase Leads to Pantothenate Overproduction in Escherichia coli Appl. Envir. Microbiol., April 1, 1999; 65(4): 1530 - 1539. [Abstract] [Full Text]
|
|
|
|
|
|
H. Peter, B. Weil, A. Burkovski, R. Krämer, and S. Morbach Corynebacterium glutamicum Is Equipped with Four Secondary Carriers for Compatible Solutes: Identification, Sequencing, and Characterization of the Proline/Ectoine Uptake System, ProP, and the Ectoine/Proline/Glycine Betaine Carrier, EctP J. Bacteriol., November 15, 1998; 180(22): 6005 - 6012. [Abstract] [Full Text]
|
|
|
|
 |
|
 F. Fieschi, E. Torrents, L. Toulokhonova, A. Jordan, U. Hellman, J. Barbe, I. Gibert, M. Karlsson, and B.-M. Sjoberg The Manganese-containing Ribonucleotide Reductase of Corynebacterium ammoniagenes Is a Class Ib Enzyme J. Biol. Chem., February 20, 1998; 273(8): 4329 - 4337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. S. M. Wösten, M. Boeve, M. G. A. Koot, A. C. van Nuenen, and B. A. M. van der Zeijst Identification of Campylobacter jejuni Promoter Sequences J. Bacteriol., February 1, 1998; 180(3): 594 - 599. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
