Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Microbiology 142 (1996), 2087-2095
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rainey, F. A.
Right arrow Articles by Stackebrandt, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rainey, F. A.
Right arrow Articles by Stackebrandt, E.
Agricola
Right arrow Articles by Rainey, F. A.
Right arrow Articles by Stackebrandt, E.

microbiology, Vol 142, 2087-2095, Copyright © 1996 by Society for General Microbiology

ARTICLES

Clostridium paradoxum DSM 7308T contains multiple 16S rRNA genes with heterogeneous intervening sequences

FA Rainey, NL Ward-Rainey, PH Janssen, H Hippe and E Stackebrandt
DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany, rainey@gbf-braunschweig.de

Sequence analysis of the cloned 16S rRNA genes of Clostridium paradoxum DSM 7308T revealed the presence of 15 different sequences in variable region I (Escherichia coli position 73-97) of the 16S rRNA. The majority of the cloned genes contained intervening sequences (IVSs), which varied in length from 120-131 nt, and were present in the DNA obtained from single colonies of C. paradoxum. The absence of IVSs in the mature rRNA was demonstrated by Northern hybridization and sequence analysis of the 16S rRNA reverse transcriptase (RT)-PCR product. This finding was supported by the failure of oligonucleotide probes specific for certain IVSs to hybridize to the RT-PCR product obtained from C. paradoxum. Alterations in culture conditions (temperature, pH, salt) or culture age did not lead to expression of RNA containing IVSs, as indicated by the size of RT-PCR products. Hybridization of the restriction-enzyme-digested genomic DNA of C. paradoxum with probes derived from the IVSs demonstrated that the 16S rRNA genes containing different IVSs are located at different sites on the chromosome.


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
J. E. Mendell, K. D. Clements, J. H. Choat, and E. R. Angert
From the Cover: Extreme polyploidy in a large bacterium
PNAS, May 6, 2008; 105(18): 6730 - 6734.
[Abstract] [Full Text] [PDF]

Home page
 Int. J. Syst. Evol. Microbiol.Home page
M. Hatamoto, H. Imachi, S. Fukayo, A. Ohashi, and H. Harada
Syntrophomonas palmitatica sp. nov., an anaerobic, syntrophic, long-chain fatty-acid-oxidizing bacterium isolated from methanogenic sludge
Int J Syst Evol Microbiol, September 1, 2007; 57(9): 2137 - 2142.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
E. A. Ottesen, J. W. Hong, S. R. Quake, and J. R. Leadbetter
Microfluidic Digital PCR Enables Multigene Analysis of Individual Environmental Bacteria
Science, December 1, 2006; 314(5804): 1464 - 1467.
[Abstract] [Full Text] [PDF]

Home page
 Int. J. Syst. Evol. Microbiol.Home page
R. U. Onyenwoke, Y.-J. Lee, S. Dabrowski, B. K. Ahring, and J. Wiegel
Reclassification of Thermoanaerobium acetigenum as Caldicellulosiruptor acetigenus comb. nov. and emendation of the genus description
Int J Syst Evol Microbiol, June 1, 2006; 56(6): 1391 - 1395.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
A. Beumer and J. B. Robinson
A Broad-Host-Range, Generalized Transducing Phage (SN-T) Acquires 16S rRNA Genes from Different Genera of Bacteria
Appl. Envir. Microbiol., December 1, 2005; 71(12): 8301 - 8304.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
C. B. Flies, J. Peplies, and D. Schuler
Combined Approach for Characterization of Uncultivated Magnetotactic Bacteria from Various Aquatic Environments
Appl. Envir. Microbiol., May 1, 2005; 71(5): 2723 - 2731.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
G. B. Rogers, M. P. Carroll, D. J. Serisier, P. M. Hockey, G. Jones, and K. D. Bruce
Characterization of Bacterial Community Diversity in Cystic Fibrosis Lung Infections by Use of 16S Ribosomal DNA Terminal Restriction Fragment Length Polymorphism Profiling
J. Clin. Microbiol., November 1, 2004; 42(11): 5176 - 5183.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
B. J. Baker, P. Hugenholtz, S. C. Dawson, and J. F. Banfield
Extremely Acidophilic Protists from Acid Mine Drainage Host Rickettsiales-Lineage Endosymbionts That Have Intervening Sequences in Their 16S rRNA Genes
Appl. Envir. Microbiol., September 1, 2003; 69(9): 5512 - 5518.
[Abstract] [Full Text] [PDF]

Home page
 Int. J. Syst. Evol. Microbiol.Home page
X. Cao, X. Liu, and X. Dong
Alkaliphilus crotonatoxidans sp. nov., a strictly anaerobic, crotonate-dismutating bacterium isolated from a methanogenic environment
Int J Syst Evol Microbiol, July 1, 2003; 53(4): 971 - 975.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
W. J. Broughton
Roses by Other Names: Taxonomy of the Rhizobiaceae
J. Bacteriol., May 15, 2003; 185(10): 2975 - 2979.
[Full Text] [PDF]

Home page
 J. Bacteriol.Home page
P. van Berkum, Z. Terefework, L. Paulin, S. Suomalainen, K. Lindstrom, and B. D. Eardly
Discordant Phylogenies within the rrn Loci of Rhizobia
J. Bacteriol., May 15, 2003; 185(10): 2988 - 2998.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
C. Teyssier, H. Marchandin, M. Simeon De Buochberg, M. Ramuz, and E. Jumas-Bilak
Atypical 16S rRNA Gene Copies in Ochrobactrum intermedium Strains Reveal a Large Genomic Rearrangement by Recombination between rrn Copies
J. Bacteriol., May 1, 2003; 185(9): 2901 - 2909.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
N. Boon, E. M. Top, W. Verstraete, and S. D. Siciliano
Bioaugmentation as a Tool To Protect the Structure and Function of an Activated-Sludge Microbial Community against a 3-Chloroaniline Shock Load
Appl. Envir. Microbiol., March 1, 2003; 69(3): 1511 - 1520.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
A. Cherif, S. Borin, A. Rizzi, H. Ouzari, A. Boudabous, and D. Daffonchio
Bacillus anthracis Diverges from Related Clades of the Bacillus cereus Group in 16S-23S Ribosomal DNA Intergenic Transcribed Spacers Containing tRNA Genes
Appl. Envir. Microbiol., January 1, 2003; 69(1): 33 - 40.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
A. M. Ibekwe, S. K. Papiernik, J. Gan, S. R. Yates, C.-H. Yang, and D. E. Crowley
Impact of Fumigants on Soil Microbial Communities
Appl. Envir. Microbiol., July 1, 2001; 67(7): 3245 - 3257.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
L. Ranjard, E. Brothier, and S. Nazaret
Sequencing Bands of Ribosomal Intergenic Spacer Analysis Fingerprints for Characterization and Microscale Distribution of Soil Bacterium Populations Responding to Mercury Spiking
Appl. Envir. Microbiol., December 1, 2000; 66(12): 5334 - 5339.
[Abstract] [Full Text]

Home page
 Appl. Environ. Microbiol.Home page
W. Dejonghe, J. Goris, S. El Fantroussi, M. Höfte, P. De Vos, W. Verstraete, and E. M. Top
Effect of Dissemination of 2,4-Dichlorophenoxyacetic Acid (2,4-D) Degradation Plasmids on 2,4-D Degradation and on Bacterial Community Structure in Two Different Soil Horizons
Appl. Envir. Microbiol., August 1, 2000; 66(8): 3297 - 3304.
[Abstract] [Full Text]

Home page
 Appl. Environ. Microbiol.Home page
J. A. Klappenbach, J. M. Dunbar, and T. M. Schmidt
rRNA Operon Copy Number Reflects Ecological Strategies of Bacteria
Appl. Envir. Microbiol., April 1, 2000; 66(4): 1328 - 1333.
[Abstract] [Full Text]

Home page
 J. Bacteriol.Home page
K. Pabbaraju, W. L. Miller, and K. E. Sanderson
Distribution of Intervening Sequences in the Genes for 23S rRNA and rRNA Fragmentation among Strains of the Salmonella Reference Collection B (SARB) and SARC Sets
J. Bacteriol., April 1, 2000; 182(7): 1923 - 1929.
[Abstract] [Full Text]

Home page
 Appl. Environ. Microbiol.Home page
A. Fernández, S. Huang, S. Seston, J. Xing, R. Hickey, C. Criddle, and J. Tiedje
How Stable Is Stable? Function versus Community Composition
Appl. Envir. Microbiol., August 1, 1999; 65(8): 3697 - 3704.
[Abstract] [Full Text]

Home page
 Appl. Environ. Microbiol.Home page
C. A. Eichner, R. W. Erb, K. N. Timmis, and I. Wagner-Döbler
Thermal Gradient Gel Electrophoresis Analysis of Bioprotection from Pollutant Shocks in the Activated Sludge Microbial Community
Appl. Envir. Microbiol., January 1, 1999; 65(1): 102 - 109.
[Abstract] [Full Text]

Home page
 Microbiol. Mol. Biol. Rev.Home page
D. M. Ward, M. J. Ferris, S. C. Nold, and M. M. Bateson
A Natural View of Microbial Biodiversity within Hot Spring Cyanobacterial Mat Communities
Microbiol. Mol. Biol. Rev., December 1, 1998; 62(4): 1353 - 1370.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
J. Wood, K. P. Scott, C. J. Newbold, and H. J. Flint
Estimation of the Relative Abundance of Different Bacteroides and Prevotella Ribotypes in Gut Samples by Restriction Enzyme Profiling of PCR-Amplified 16S rRNA Gene Sequences
Appl. Envir. Microbiol., October 1, 1998; 64(10): 3683 - 3689.
[Abstract] [Full Text]

Home page
 Appl. Environ. Microbiol.Home page
M. F. de los Reyes, F. L. de los Reyes III, M. Hernandez, and L. Raskin
Quantification of Gordona amarae Strains in Foaming Activated Sludge and Anaerobic Digester Systems with Oligonucleotide Hybridization Probes
Appl. Envir. Microbiol., July 1, 1998; 64(7): 2503 - 2512.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1996 Society for General Microbiology.