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Attachment of Vibrio alginolyticus to chitin mediated by chitin-binding proteins

Istituto di Microbiologia, Facoltà di Medicina, Università di Ancona, Ancona 60131, Italy
Istituto di Microbiologia, Università di Genova, Genoa 16132, Italy
Cattedra di Planctologia Istituto di Scienze Ambientali Marine, Università di Genova, Genoa 16132, Italy

⁴Author for correspondence: Carla Pruzzo. Tel: +39 71 2204697. Fax: +39 71 2204693.

ABSTRACT

Vibrio alginolyticus is the only culturable vibrio associated with the chitinaceous carapace of the copepod Tigriopus fulvus (Fisher 1860) living in Ligurian coastal rock pools (Tyrrhenian Sea). The characteristics of the interaction between chitin particles and V. alginolyticus were studied by analysing strains isolated both from the copepod surface and from rock-pool water. The highest degree of attachment to chitin was observed at 20°, in the presence of 3% NaCI. Bacterial treatment with N-acetylglucosamine and pronase E caused a reduction in attachment of 52-62% and 77-94%, respectively. Chitin pretreatment with either wheat germ agglutinin or membrane proteins (MPs) from V. alginolyticus caused a reduction in attachment, of 50-57% and 53-70%, respectively. No inhibition was observed when bacteria were pretreated with D-glucose, D-fucose or D-fructose, or when chitin was pretreated with concanavalin A and Escherichia coli DH5 MPs. V. alginolyticus MPs able to bind chitin were isolated and analysed by SDS-PAGE. Four chitin-binding proteins were visualized in all tested strains (53, 35, 20 and 14 kDa); in vivo these peptides may efficiently mediate V. alginolyticus attachment to chitin-containing substrates.

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