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Microbiology 142 (1996), 2223-2226
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microbiology, Vol 142, 2223-2226, Copyright © 1996 by Society for General Microbiology

ARTICLES

Endophytic taxol-producing fungi from bald cypress, Taxodium distichum

JY Li, G Strobel, R Sidhu, WM Hess and EJ Ford
Department of Plant Pathology, Montana State University, Bozeman 59717, USA.

Pestalotiopsis microspora occurs as a range of strains in bald cypress, Taxodium distichum. The organisms live as endophytes in the bark, phloem and xylem, and isolates show differences in cultural and microscopic characteristics on common laboratory media. Many of these fungi make taxol as determined by the reactivity of partially purified culture extracts with specific monoclonal antibodies against taxol. In the case of one strain of P. microspora (CP-4), taxol was isolated from culture medium and was shown to be identical to authentic taxol by chromatographic and spectroscopic means.


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