HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Stoll, R. Articles by Ferguson, S. J. Search for Related Content PubMed PubMed Citation Articles by Stoll, R. Articles by Ferguson, S. J. Agricola Articles by Stoll, R. Articles by Ferguson, S. J.

Cytochrome c₅₅₀ expression in Paracoccus denitrificans strongly depends on growth condition: identification of promoter region for cycA by transcription start analysis

Raphael Stoll^1,

Yoshihiro Sambongi^1,

Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK

²Author for correspondence: Stuart J. Ferguson. Tel: +44 1865 275240. Fax: +44 1865 275259. e-mail: ferguson@bioch.ox.ac.uk

ABSTRACT

The periplasmic cytochrome c₅₅₀ content of Paracoccus denitrificans has been shown by immunological detection to be strongly dependent on the mode of growth. Cells grown under anaerobic, denitrifying conditions or methylotrophically in the presence of oxygen contained substantially more cytochrome c₅₅₀ than cells grown aerobically on multicarbon substrates. A similar pattern was observed when expression of the cycA gene (encoding cytochrome c₅₅₀), was monitored using an Escherichia coli alkaline phosphatase gene (phoA) fusion as a reporter of cycA promoter activity. The increase in cycA expression observed during growth on C₁ substrates was substantially diminished if succinate was also present. These results reveal that expression of cycA is subject to multiple regulatory controls and suggest that cytochrome c₅₅₀ has a general role in electron transfer to periplasmic reductases required for anaerobic denitrifying growth and from dehydrogenases required for aerobic growth on C₁ compounds. Two major transcriptional initiation start points for the cycA gene have been identified.

Present address: Physiologisch-chemisches Institut der Universitat, Hoppe-Seyler Str. 4, D-72076 Tubingen, Germany.

Present address: ISIR, Osaka University, Mihogaoka 8-1, Ibaraki, Osaka 567, Japan.

This article has been cited by other articles:

				M. F. Olmo-Mira, M. Gavira, D. J. Richardson, F. Castillo, C. Moreno-Vivian, and M. D. Roldan NapF Is a Cytoplasmic Iron-Sulfur Protein Required for Fe-S Cluster Assembly in the Periplasmic Nitrate Reductase J. Biol. Chem., November 26, 2004; 279(48): 49727 - 49735. [Abstract] [Full Text] [PDF]

				G. Butland, S. Spiro, N. J. Watmough, and D. J. Richardson Two Conserved Glutamates in the Bacterial Nitric Oxide Reductase Are Essential for Activity but Not Assembly of the Enzyme J. Bacteriol., January 1, 2001; 183(1): 189 - 199. [Abstract] [Full Text]

				T. Yano and T. Yagi H+-translocating NADH-Quinone Oxidoreductase (NDH-1) of Paracoccus denitrificans. STUDIES ON TOPOLOGY AND STOICHIOMETRY OF THE PERIPHERAL SUBUNITS J. Biol. Chem., October 1, 1999; 274(40): 28606 - 28611. [Abstract] [Full Text] [PDF]

				S. C. Baker, S. J. Ferguson, B. Ludwig, M. D. Page, O.-M. H. Richter, and R. J. M. van Spanning Molecular Genetics of the Genus Paracoccus: Metabolically Versatile Bacteria with Bioenergetic Flexibility Microbiol. Mol. Biol. Rev., December 1, 1998; 62(4): 1046 - 1078. [Abstract] [Full Text] [PDF]