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The Lipid a Biosynthesis Deficiency of the Escherichia Coli Antibiotic-Supersensitive Mutant LH530 is Suppressed by a Novel Locus, ORF195

¹Department of Bacteriology and Immunology, Haartman Institute, PO Box 21 (Haartmaninkatu 3), SF-00014 University of Helsinki, Helsinki, Finland
²Department of Bacterial Vaccine Research and Molecular Biology, National Public Health Institute, Mannerheimintie 166, FIN-00300 Helsinki, Finland

ABSTRACT

A new mutant of Escherichia coli K-12 supersensitive to both hydrophobic and large hydrophilic antibiotics was isolated and characterized. The mutant grew well at 28 °C, poorly at 37 °C, and did not grow at 42 °C. The rate of its lipid A biosynthesis was reduced as compared to that of the parent strain. This deficiency was rescued by a novel locus, ORF195, the function of which has not been elucidated. ORF195 is located in the 76 min region in the E. coli chromosome and encodes a hypothetical 21.8 kDa protein with no signal sequence. ORF195 isolated from the mutant strain had an identical sequence to the wild-type allele, indicating a suppressor function of the gene product.

³Author for correspondence: Laura Hirvas. Fax: + 358 4346382.

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