HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by McIntosh, D. Articles by Villena, A. J. Search for Related Content PubMed PubMed Citation Articles by McIntosh, D. Articles by Villena, A. J. Agricola Articles by McIntosh, D. Articles by Villena, A. J.

Production of putative virulence factors by Renibacterium salmoninarum grown in cell culture

D. McIntosh^1,

Department of Biological Sciences, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, UK
Department of Biological Sciences, University of Plymouth, Plymouth PL4 8AA, UK
Departamento de Biología Celular y Anatomía, Facultad de Biología, Universidad de León, 24071-León, Spain

¹ Author for correspondence: B. Austin. Tel: + 44 131 451 3452. Fax: +44 131 451 3009. e-mail: B. Austin@hw.ac.uk

ABSTRACT

A cell culture system, employing the fish cell line Epithelioma papillosum cyprini (EPC), was developed to study the synthesis of intracellular antigen and the expression of putative virulence factors by Renibacterium salmoninarum. EPC cultures infected with R. salmoninarum could be maintained for 7 weeks, during which the pathogen multiplied intracellularly. Immunohistochemical examination of infected cultures revealed the production of the p57 antigen, haemolysin and cytolysin. The intracellular nature of the infection was confirmed by transmission electron microscopic examination of EPC monolayers. A comparison of the relative virulence of bacterial cells cultured in EPC cells and on agar plates revealed that the former were markedly more virulent in challenge experiments with juvenile rainbow trout (Oncorhynchus mykiss Walbaum). The EPC cell culture model provided a system for the study of R. salmoninarum under more natural conditions than those achieved with plate culture techniques.

Present address: Departamento Bioquimica e Biologia Molecular, Instituto Oswaldo Cruz, FIOCRUZ, Avenida Brasil 4365, Rio de Janeiro, 21045 900, Brazil.

This article has been cited by other articles:

				G. D. Wiens, D. D. Rockey, Z. Wu, J. Chang, R. Levy, S. Crane, D. S. Chen, G. R. Capri, J. R. Burnett, P. S. Sudheesh, et al. Genome Sequence of the Fish Pathogen Renibacterium salmoninarum Suggests Reductive Evolution away from an Environmental Arthrobacter Ancestor J. Bacteriol., November 1, 2008; 190(21): 6970 - 6982. [Abstract] [Full Text] [PDF]

				L. D. Rhodes, A. M. Coady, and R. K. Deinhard Identification of a Third msa Gene in Renibacterium salmoninarum and the Associated Virulence Phenotype Appl. Envir. Microbiol., November 1, 2004; 70(11): 6488 - 6494. [Abstract] [Full Text] [PDF]