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1Microbiology Group, International Centre for Genetic Engineering and Biotechnology and ITALTB5 Ricerche, Sri, AREA Science Park, Padriciano 99, 1-34012 Trieste, Italy
2Department of Genetics and Microbiology ‘A. Buzzati Traverso’, University of Pavia, 27100 Pavia, Italy
3Author for correspondence: Carlo V. Bruschi. Tel: +39 40 375 7304. Fax: +59 40 375 7343. e-mail: bruschi@icgeb.trieste.it
ABSTRACT
A 15 kb DNA fragment from the Bacillus subtilis chromosome between citB and ppsC has been sequenced, and new ORFs encoding putative enzymes involved in lipopolypeptide synthesis, which complete a partial operon previously reported, and a new set of enzymes responsible for lipid metabolism have been identified. From the analysis of DNA sequence homology of the fragment it was deduced that these new peptide synthetase genes are part of an operon for the biosynthesis of the fungicide fengycin.
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