HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kormanec, J. Articles by Homérová, D. Search for Related Content PubMed PubMed Citation Articles by Kormanec, J. Articles by Homérová, D. Agricola Articles by Kormanec, J. Articles by Homérová, D.

Expression of the Streptomyces aureofaciens glyceraldehyde-3-phosphate dehydrogenase gene (gap) is developmentally regulated and induced by glucose

B. euchová

Institute of Molecular Biology, Slovak Academy of Sciences, 842 51 Bratislava, Slovak Republic

¹ Author for correspondence: J. Kormanec. Tel: + 42 1 7 378 2432. Fax: +42 1 7 372 316. e-mail: umbijkor@savba.sk

ABSTRACT

In previous experiments, the Streptomyces aureofaciens gap gene encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was identified. To investigate expression of the gene, S1 nuclease mapping and Northern blot hybridization were performed using RNA prepared from S. aureofaciens cultivated under various conditions. These studies suggested monocistronic organization and developmental regulation of the gene. A single promoter, gap-P, was identified upstream of the gap coding region. In cultures grown on solid medium in the absence of glucose, its transcription was induced at the time of aerial mycelium formation. In addition, gap transcription was also induced in substrate mycelium by glucose. A promoter-bearing DNA fragment was inserted into two promoter-probe vectors, to give expression patterns consistent with the results of direct RNA analysis.

This article has been cited by other articles:

				G.-T. Lu, J.-R. Xie, L. Chen, J.-R. Hu, S.-Q. An, H.-Z. Su, J.-X. Feng, Y.-Q. He, B.-L. Jiang, D.-J. Tang, et al. Glyceraldehyde-3-phosphate dehydrogenase of Xanthomonas campestris pv. campestris is required for extracellular polysaccharide production and full virulence Microbiology, May 1, 2009; 155(5): 1602 - 1612. [Abstract] [Full Text] [PDF]

				L. D. Nguyen, L. Kalachova, J. Novotna, M. Holub, O. Kofronova, O. Benada, C. J. Thompson, and J. Weiser Cultivation System Using Glass Beads Immersed in Liquid Medium Facilitates Studies of Streptomyces Differentiation Appl. Envir. Microbiol., June 1, 2005; 71(6): 2848 - 2852. [Abstract] [Full Text] [PDF]

				O. Sprusanský, B. Rezuchová, D. Homerová, and J. Kormanec Expression of the gap gene encoding glyceraldehyde-3-phosphate dehydrogenase of Streptomyces aureofaciens requires GapR, a member of the AraC/XylS family of transcriptional activators Microbiology, May 1, 2001; 147(5): 1291 - 1301. [Abstract] [Full Text]

				S. Fillinger, S. Boschi-Muller, S. Azza, E. Dervyn, G. Branlant, and S. Aymerich Two Glyceraldehyde-3-phosphate Dehydrogenases with Opposite Physiological Roles in a Nonphotosynthetic Bacterium J. Biol. Chem., May 5, 2000; 275(19): 14031 - 14037. [Abstract] [Full Text] [PDF]