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Microbiology 143 (1997), 3563-3571; DOI  10.1099/00221287-143-11-3563
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Streptomyces lividans groES, groEL1 and groEL2 genes

Patricia de León1, Sergio Marco1, Carolina Isiegas1, Anabel Marina2, José L. Carrascosa1 and Rafael P. Mellado1,3

1Centro Nacional de Biotecnología (CSIC), Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain
2Centra de Biología Molecular Severo Ochoa, Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain

3Author for correspondence: Rafael P. Mellado. Tel: +34 1 5854547. Fax: +34 1 5S54506. e-mail: rpmellado@samba.cnb.uam.es

ABSTRACT

The Streptomyces lividans groES/ELI operon and groEL2 gene were cloned and their respective DNA sequences determined. The sequenced DNA comprised the genes and their respective regulatory regions in both cases. Transcription of both groES/EL1 and groEL2 seemed to be subjected to temporal control at 30 °C. At 45 °C the amount of the groEL2 transcript increased considerably in comparison to that of groES/EL1. Among the proteins synthesized under heat shock by S. lividans, a fraction enriched in GroEL2 showed the presence of a ring-shaped structure that resembles that of other chaperonins and was active in a rhodanase folding assay.

Keywords: Streptomyces lividans, GroEL-like chaperones, hear shock




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