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Manipulation of the physiology of clavulanic acid production in Streptomyces clavuligerus

Paul R. Ives

Microbial Physiology and Ecology Group, University of Surrey, Guildford, Surrey GU2 5XH, UK

¹ Author for correspondence: Michael L Bushel]. Tel: +44 148.3 259277. Fax: +44 1483 300374. e-mail: m.hushell@surrey.ac.uk

ABSTRACT

This paper reports a novel use of cluster analysis for the identification of intermediary metabolites that are produced at rates closely correlated with those of antibiotic biosynthesis. This information was used to devise culture feeds resulting in enhanced production of clavulanic acid, an antibiotic of current worldwide commercial interest. The feeding strategies apparently alleviated a rate-limiting supply of the C₃ precursor of clavulanic acid. C₃ limitation may be a consequence of unusual nitrogen and carbon metabolism in Streptomyces clavuligerus. This approach has potential as a generic method for influencing biosynthetic pathway fluxes using feeds without knowledge of the biosynthetic pathway.

Present address: Gist-brocades BV, PO Box 1, 2600 MA Delft, The Netherlands.

This article has been cited by other articles:

				F. Voelker and S. Altaba Nitrogen source governs the patterns of growth and pristinamycin production in 'Streptomyces pristinaespiralis' Microbiology, September 1, 2001; 147(9): 2447 - 2459. [Abstract] [Full Text] [PDF]