HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Charland, N. Articles by Gottschalk, M. Search for Related Content PubMed PubMed Citation Articles by Charland, N. Articles by Gottschalk, M. Agricola Articles by Charland, N. Articles by Gottschalk, M.

Characterization and protective activity of a monoclonal antibody against a capsular epitope shared by Streptococcus suis serotypes 1, 2 and 1/2

Groupe de Recherche sur les Maladies Infectieuses du Pore, Faculté de médécine vétérinaire, Université de Montréal, CP 5000 St-Hyacinthe, Québec, Canada J2S 7C6

¹ Author for correspondence: Marcelo Gottschalk. Tel: +1 514 773 8521 ext. 8374. Fax: + 1 514 778 8108. e-mail: gottschm@ere.umontreal.ca

ABSTRACT

A monoclonal antibody (mAb Z3) was produced using BALB/c mice immunized with whole cells of Streptococcus suis serotype 2 reference strain S735. Screening by dot-ELISA showed that mAb Z3, of isotype lgG2b, reacted only with reference strains and field isolates of S. suis serotypes 1, 2 and 1/2. The recognized epitope was demonstrated to be polysaccharide in nature by periodate oxidation, and located in the capsule, since mAb Z3 reacted with purified capsular material by immunoblotting and was able to stabilize the capsule as shown by electron microscopy. Further characterization indicated that mAb Z3 may react specifically with the sialic acid moiety of the capsule, a common constituent of the polysaccharidic capsular material of the three capsular types, since sialidase-treated cells did not react with mAb Z3 in immunoblotting or indirect EILISA. Purified mAb Z3 was shown to significantly increase the rate of phagocytosis of S. suis cells by porcine monocytes and to activate the clearance of bacteria from the circulation in experimentally infected mice. However, mAb Z3 only offered partial protection to mice challenged with a minimal lethal dose. Thus, even though the capsule of S. suis seems to be an important virulence factor, the epitope recognized by mAb Z3 does not appear to be involved in complete protection against infection.

This article has been cited by other articles:

				M. Segura and M. Gottschalk Streptococcus suis Interactions with the Murine Macrophage Cell Line J774: Adhesion and Cytotoxicity Infect. Immun., August 1, 2002; 70(8): 4312 - 4322. [Abstract] [Full Text] [PDF]

				H. E. Smith, V. Veenbergen, J. van der Velde, M. Damman, H. J. Wisselink, and M. A. Smits The cps Genes of Streptococcus suis Serotypes 1, 2, and 9: Development of Rapid Serotype-Specific PCR Assays J. Clin. Microbiol., October 1, 1999; 37(10): 3146 - 3152. [Abstract] [Full Text]

				M. Gottschalk, S. Lacouture, and L. Odierno Immunomagnetic Isolation of Streptococcus suis Serotypes 2 and 1/2 from Swine Tonsils J. Clin. Microbiol., September 1, 1999; 37(9): 2877 - 2881. [Abstract] [Full Text]