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A physiological model for the control of erythromycin production in batch and cyclic fed batch culture

J. Smith^1,

H. C. Lynch^1,

Microbial Products Laboratory, Biological Sciences, University of Surrey, Guildford, Surrey, GU2 5XH, UK

²Author for correspondence: M. E. Bushell. Tel: + 44 1483 259277. Fax: +44 14S3 300374. e-mail: m.bushell@surrey.ac.uk

ABSTRACT

Reported differences in antibiotic production dynamics resulting from altering the growth-limiting nutrient (growth-dissociated production in carbon-limited culture and apparent growth-associated production in nitrogen-limited culture) are due to the different effects on growth kinetics. The substrate affinity for nitrate is significantly lower than that for glucose, resulting in nitrogen limitation effectively occurring throughout the culture. Glucose limitation occurs later in the culture, coinciding with the induction of antibiotic production. Induction occurs at the start of nitrogen-limited culture so that production appears to be growth-associated. Evidence that this hypothesis is consistent with production kinetics in cyclic fed batch culture was also obtained.

Present address: Food Science and Technology, University of Reading, Whiteknights, PO Box 226, Reading RG6 2AP, UK.

Present address: Biotechnology Research, Whitbread Technical Centre, Park Street, Luton LU1 5SR, UK.