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The recA gene from Clostridium perfringens is induced by methyl methanesulphonate and contains an upstream Cheo box

Department of Microbiology, Monash University, Clayton, Victoria 3168, Australia

ABSTRACT

The recA gene from Clostridium perfringens was cloned using degenerate oligonucleotide primers designed from conserved regions of RecA proteins from other bacteria. The 1089 bp gene encoded a putative RecA protein with 69% amino acid sequence similarity to the RecA protein from Bacillus subtilis. The C. perfringens recA gene was induced by exposure to methyl methanesulphonate and complemented a recA mutant of Escherichia coli. A Cheo box was identified in the region upstream of the gene. Since this SOS-like operator site is conserved in many DNA-damage-inducible recA gene regions from Gram-positive bacteria, the results suggest that the regulation of the C. perfringens recA gene also involves the binding of a LexA-like protein to this site.

^*Author for correspondence: Julian I. Rood. Tel: + 61 3 9905 4825. Fax: + 61 3 9905 4811. e-mail: julian.rood@med.monash.edu.au

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