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K processing
Istituto di Scienze dell'Alimentazione, Consiglio Nazionale delle Ricerche, via Roma, 83100 Avellino, Italy
Dipartimento di Chimica, Università degli Studi di Salerno, via Ponte Don Melillo, 84084 Fisciano (Sa), Italy
Sezione di Microbiologia, Dipartimento di Fisioiogia Generale ed Ambientale, Universitá Federico II, via Mezzocannone 16, 80134 Naples, Italy
Istituto Internazionale di Genetica e Biofisica, Consiglio Nazionale delle Ricerche, via G. Marconi 10, 80125 Naples, Italy
Author for correspondence: Margherita Sacco. Tel: + 39 81 7257219. Fax: + 39 81 5936123. e-mail: sacco@:iigbna.iigbna.cnr.it
ABSTRACT
The Bacillus subtilis BofA protein is involved in regulation of pro-
K processing in the mother cell during the late stages of sporulation. A computer analysis of the BofA amino acid sequence indicates that it is an integral membrane protein. To determine the membrane topology of the protein, a series of gene fusions of bofA with lacZ or phoA reporter genes in Escherichia coli were analysed. A BofA topological model with two membrane-spanning segments, and with the N- and the C-terminal domains located in the region between the inner and outer membranes surrounding the forespore is presented. The analysis of different modifications of the last five amino acid residues of the BofA protein, obtained by PCR site-directed mutagenesis, suggests a possible role of the C-terminal domain in the regulation of pro-K processing.
K, processing, gene fusion, mutagenesis, Bacillus subtilisThis article has been cited by other articles:
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L. Kroos, Y.-T. N. Yu, D. Mills, and S. Ferguson-Miller Forespore Signaling Is Necessary for Pro-{sigma}K Processing during Bacillus subtilis Sporulation Despite the Loss of SpoIVFA upon Translational Arrest J. Bacteriol., October 1, 2002; 184(19): 5393 - 5401. [Abstract] [Full Text] [PDF]
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D. H. Green and S. M. Cutting Membrane Topology of the Bacillus subtilis Pro-sigma K Processing Complex J. Bacteriol., January 15, 2000; 182(2): 278 - 285. [Abstract] [Full Text]
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O. Resnekov Role of the Sporulation Protein BofA in Regulating Activation of the Bacillus subtilis Developmental Transcription Factor sigma K J. Bacteriol., September 1, 1999; 181(17): 5384 - 5388. [Abstract] [Full Text]
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