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A 12 kb nucleotide sequence containing the alanine dehydrogenase gene at 279° on the Bacillus subtilis chromosome

¹Department of Molecular Microbiology, Institute of Molecular Biological Sciences, BioCentrum Amsterdam, Faculty of Biology, Vrije Universiteit, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands
²Department of Microbiology, Faculty of Agronomy, 6 Avenue du Maréchal Juin, B-5030 Gembloux, Belgium

ABSTRACT

In the framework of the European project aimed at the sequencing of the Bacillus subtilis genome, a DNA fragment of 12315 bp was cloned and sequenced. The DNA fragment is located between rrnB (275°) and pai (284°). Twelve ORFs were predicted to encode putative proteins. Two of these (aid and yukl) coincided with known B. subtilis genes. The products of two other genes (yukK and yukL) showed significant similarity to known proteins present in databases, e.g. pyoverdine synthase of Pseudomonas aeruginosa and pristinamycine synthase D of Streptomyces pristinaespiralis.

Author for correspondence: Bauke Oudega. Tel: +31 20 4447177. Fax: +31 20 4447229. e-mail: oudega@bio.vu.nl

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