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Microbiology 143 (1997), 1583-1586
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microbiology, Vol 143, 1583-1586, Copyright © 1997 by Society for General Microbiology


ARTICLES

Inhibition of Paracoccidioides brasiliensis by ajoene is associated with blockade of phosphatidylcholine biosynthesis [published erratum appears in Microbiology 1998 Jan;144 (Pt 1):261]

G San-Blas, JA Urbina, E Marchan, LM Contreras, F Sorais and F San-Blas
Instituto Venezolano de Investigaciones Cientificas (IVIC), Centres of Microbiology and Cell Biology, Caracas, Venezuela. gsanblas@pasteur.ivic.ve

In Paracoccidioides brasiliensis, a dimorphic fungus pathogenic for humans, no significant differences were observed in the phospholipid species of both morphological phases. The species observed were phosphatidylcholine (PC, 30-40%), phosphatidylethanolamine (PE, 27- 28%), phosphatidylserine (16-19%), phosphatidylinositol (13-17%) and sphingomyelin (3-5%). The main fatty acids found in the yeast (Y) phase were palmitate (56%), linoleate (18%) and oleate (15%), while linoleate predominated (61%) in the mycelial (M) phase, followed by palmitate (27%) and oleate (7%). In the Y phase the main free sterol was ergosta- 5,22-dien-3 beta-ol (82%) plus some lanosterol (12%) and ergosterol (6%), while in the M phase, the latter predominated (88%), followed by low levels of ergosta-5,22-dien-3 beta-ol (12%). Ajoene [(E,Z)-4,5,9- trithiadodeca-1,6,11-triene 9-oxide], a platelet aggregation inhibitor derived from garlic, induced alterations in phospholipid and fatty acid proportions such that PC was reduced to about 18% in both phases and PE increased to 38% (Y phase) or 44% (M phase), suggesting inhibition of PC synthesis. Ajoene also reduced saturated fatty acids (16:0 and 18:0) from 67 to 35% in the Y phase, with a corresponding increase in the unsaturated components. This effect was not seen in the M phase.


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Antimicrob. Agents Chemother.Home page
G. Visbal, A. Alvarez, B. Moreno, and G. San-Blas
S-Adenosyl-L-Methionine Inhibitors {Delta}24-Sterol Methyltransferase and {Delta}24(28)-Sterol Methylreductase as Possible Agents against Paracoccidioides brasiliensis
Antimicrob. Agents Chemother., September 1, 2003; 47(9): 2966 - 2970.
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