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Microbiology 143 (1997), 1583-1586; DOI  10.1099/00221287-143-5-1583
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Inhibition of Paracoccidioides brasiliensis by ajoene is associated with blockade of phosphatidylcholine biosynthesis

Gioconda San-Bias1, Julio A. Urbina2, Edgar Marchán2, Lellys M. Contreras2, Françoise Sorais1 and Felipe San-Blas1

1Institute Venezolano de Investigaciones Científicas (IVIC), Centres of Microbiology and Cell Biology, Caracas 1020A, Venezuela
2Biophysics and Biochemistry Apartado 21827, Caracas 1020A, Venezuela

ABSTRACT

In Paracoccidioides brasiliensis, a dimorphic fungus pathogenic for humans, no significant differences were observed in the phospholipid species of both morphological phases. The species observed were phosphatidylcholine (PC, 30-40%), phosphatidylethanolamine (PE, 27-28%), phosphatidylserine (16-19%), phosphatidylinositol (13-17%) and sphingomyelin (3-5%). The main fatty acids found in the yeast (Y) phase were palmitate (56%), linoleate (18%) and oleate (15%), while linoleate predominated (61 %) in the mycelial (M) phase, followed by palmitate (27%) and oleate (7%). In the Y phase the main free sterol was ergosta-5,22-dien-3β-ol (82%) plus some lanosterol (12%) and ergosterol (6%), while in the M phase, the latter predominated (88%), followed by low levels of ergosta-5,22-dien-3β-ol (12%). Ajoene [(E,Z)-4,5,9-trithiadodeca-1,6,11-triene 9-oxide], a platelet aggregation inhibitor derived from garlic, induced alterations in phospholipid and fatty acid proportions such that PC was reduced to about 18% in both phases and PE increased to 38% (Y phase) or 44% (M phase), suggesting inhibition of PC synthesis. Ajoene also reduced saturated fatty acids (16:0 and 18:0) from 67 to 35% in the Y phase, with a corresponding increase in the unsaturated components. This effect was not seen in the M phase.

Author for correspondence: Gioconda San-Bias. Tel: +58 2 504 1040. Fax: +58 2 504 1382. e-mail: gsanblas@pasteur.ivic.ve


Keywords: fungal dimorphism, Paracoccidioides brasiliensis, ajoene, antifungal drugs, fungal lipids




This article has been cited by other articles:


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Antimicrob. Agents Chemother.Home page
G. Visbal, A. Alvarez, B. Moreno, and G. San-Blas
S-Adenosyl-L-Methionine Inhibitors {Delta}24-Sterol Methyltransferase and {Delta}24(28)-Sterol Methylreductase as Possible Agents against Paracoccidioides brasiliensis
Antimicrob. Agents Chemother., September 1, 2003; 47(9): 2966 - 2970.
[Abstract] [Full Text] [PDF]




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