Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Microbiology 143 (1997), 1583-1586
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by San-Blas, G.
Right arrow Articles by San-Blas, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by San-Blas, G.
Right arrow Articles by San-Blas, F.
Agricola
Right arrow Articles by San-Blas, G.
Right arrow Articles by San-Blas, F.

microbiology, Vol 143, 1583-1586, Copyright © 1997 by Society for General Microbiology

ARTICLES

Inhibition of Paracoccidioides brasiliensis by ajoene is associated with blockade of phosphatidylcholine biosynthesis [published erratum appears in Microbiology 1998 Jan;144 (Pt 1):261]

G San-Blas, JA Urbina, E Marchan, LM Contreras, F Sorais and F San-Blas
Instituto Venezolano de Investigaciones Cientificas (IVIC), Centres of Microbiology and Cell Biology, Caracas, Venezuela. gsanblas@pasteur.ivic.ve

In Paracoccidioides brasiliensis, a dimorphic fungus pathogenic for humans, no significant differences were observed in the phospholipid species of both morphological phases. The species observed were phosphatidylcholine (PC, 30-40%), phosphatidylethanolamine (PE, 27- 28%), phosphatidylserine (16-19%), phosphatidylinositol (13-17%) and sphingomyelin (3-5%). The main fatty acids found in the yeast (Y) phase were palmitate (56%), linoleate (18%) and oleate (15%), while linoleate predominated (61%) in the mycelial (M) phase, followed by palmitate (27%) and oleate (7%). In the Y phase the main free sterol was ergosta- 5,22-dien-3 beta-ol (82%) plus some lanosterol (12%) and ergosterol (6%), while in the M phase, the latter predominated (88%), followed by low levels of ergosta-5,22-dien-3 beta-ol (12%). Ajoene [(E,Z)-4,5,9- trithiadodeca-1,6,11-triene 9-oxide], a platelet aggregation inhibitor derived from garlic, induced alterations in phospholipid and fatty acid proportions such that PC was reduced to about 18% in both phases and PE increased to 38% (Y phase) or 44% (M phase), suggesting inhibition of PC synthesis. Ajoene also reduced saturated fatty acids (16:0 and 18:0) from 67 to 35% in the Y phase, with a corresponding increase in the unsaturated components. This effect was not seen in the M phase.


This article has been cited by other articles:


Home page
 Antimicrob. Agents Chemother.Home page
G. Visbal, A. Alvarez, B. Moreno, and G. San-Blas
S-Adenosyl-L-Methionine Inhibitors {Delta}24-Sterol Methyltransferase and {Delta}24(28)-Sterol Methylreductase as Possible Agents against Paracoccidioides brasiliensis
Antimicrob. Agents Chemother., September 1, 2003; 47(9): 2966 - 2970.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1997 Society for General Microbiology.