HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Montijn, R. C. Articles by Klis, F. M. Search for Related Content PubMed PubMed Citation Articles by Montijn, R. C. Articles by Klis, F. M. Agricola Articles by Montijn, R. C. Articles by Klis, F. M.

β-Glucosylated proteins in the cell wall of the black yeast Exophiala (Wangiella) dermatitidis

¹Institute of Molecular Cell Biology, University of Amsterdam, Biocentrum Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands
²Centraalbureau voor Schimmelcultures, PO Box 273, Baarn, The Netherlands

ABSTRACT

Wild-type cells of the pathogenic black yeast Exophiala (Wangiella) dermatitidis grown in a low-pH ascorbate medium became less melanized and less resistant to Zymolyase. This was accompanied by increased staining with fluorescently labelled concanavalin A. The sugar composition of wild-type and mutant cell walls was, except for the presence of galactose, similar to that of Saccharomyces cerevisiae. Digestion of mutant cell walls with laminarinase released galactomannoproteins. In addition, the released cell wall proteins contained glucose and reacted with affinity-purified 1,6-β-glucan antiserum, indicating that they are linked to 1,6-β-glucan. It is proposed that 1,6-β-glucosylated cell wall proteins generally occur among ascomycetes.

Author for correspondence: Roy C. Montijn. Tel: +31 20 525 7843. Fax: +31 20 525 7834 e-mail: montijn@.bio.uva.nl

This article has been cited by other articles:

				H. Liu, S. Kauffman, J. M. Becker, and P. J. Szaniszlo Wangiella (Exophiala) dermatitidis WdChs5p, a Class V Chitin Synthase, Is Essential for Sustained Cell Growth at Temperature of Infection Eukaryot. Cell, February 1, 2004; 3(1): 40 - 51. [Abstract] [Full Text] [PDF]

				Q. Wang, H. Liu, and P. J. Szaniszlo Compensatory expression of five chitin synthase genes, a response to stress stimuli, in Wangiella (Exophiala) dermatitidis, a melanized fungal pathogen of humans Microbiology, September 1, 2002; 148(9): 2811 - 2817. [Abstract] [Full Text] [PDF]

				B. Feng, X. Wang, M. Hauser, S. Kaufmann, S. Jentsch, G. Haase, J. M. Becker, and P. J. Szaniszlo Molecular Cloning and Characterization of WdPKS1, a Gene Involved in Dihydroxynaphthalene Melanin Biosynthesis and Virulence in Wangiella (Exophiala) dermatitidis Infect. Immun., March 1, 2001; 69(3): 1781 - 1794. [Abstract] [Full Text] [PDF]

				N. Schnitzler, H. Peltroche-Llacsahuanga, N. Bestier, J. Zundorf, R. Lutticken, and G. Haase Effect of Melanin and Carotenoids of Exophiala (Wangiella) dermatitidis on Phagocytosis, Oxidative Burst, and Killing by Human Neutrophils Infect. Immun., January 1, 1999; 67(1): 94 - 101. [Abstract] [Full Text] [PDF]

				M. Lussier, A.-M. Sdicu, S. Shahinian, and H. Bussey The Candida albicans KRE9 gene is required for cell wall beta -1,6-glucan synthesis and is essential for growth on glucose PNAS, August 18, 1998; 95(17): 9825 - 9830. [Abstract] [Full Text] [PDF]

				P. N. Lipke and R. Ovalle Cell Wall Architecture in Yeast: New Structure and New Challenges J. Bacteriol., August 1, 1998; 180(15): 3735 - 3740. [Full Text]