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1 Environmental Engineering Science, W. M. Keck Laboratories 138-78, California Institute of Technology, Pasadena, CA91125, USA
2 Institute of Genetics and Selection of Industrial Microorganisms, 1 Dorozhniy pr. T, Moscow 114570, Russia
3 Marine Sciences Research Center, SUNY at Stony Brook, Stony Brook, NY 11794-5000, USA
Author for correspondence: Andrei Y. Chistoserdov. Tel: +1 516 632 9233. Fax: +1 516 632 8820. e-mail: chistoserdov@ccmail.sunysb.edu
ABSTRACT
The organization of genes involved in utilization of methylamine (mau genes) was studied in the obligate methylotroph ‘Methylobacillus flagellatum’ KT. Nine open reading frames were identified as corresponding to the genes mauFBEDAGLMN. In addition, an open reading frame (orf-1) encoding a polypeptide with unknown function was identified upstream of the mau gene cluster. Subclones of the ‘M. flagellatum’ KT gene cluster were used for complementation of a series of chemically induced mau mutants of ‘M. flagellatum’ KT. Mutants in mauF, mauB, mauEID, mauA, mauG, mauL and mauM were identified. Two mutants (mau-18 and mau-19) were not complemented by the known mau genes. Since none of the chemically induced mutants studied had a defect in orf-1 or mauN, insertion mutants in these genes were constructed. Phenotypically the mutants fell into three groups. The mauF, mauB, mauE/D, mauA, mauG, mauL and mauM mutants do not grow on methylamine as a source of carbon and lack methylamine dehydrogenase activity, but they synthesize both the large and the small subunit polypeptides albeit at different ratios. The mau-18 and mau-19 mutants do not grow on methylamine as a source of carbon, and lack both methylamine dehydrogenase activity and the methylamine dehydrogenase subunits. The orf-1 and mauN mutants grow on methylamine as a source of carbon and synthesize wild-type levels of methylamine dehydrogenase. It has been shown earlier that the product of the mauM gene is not required for synthesis of active methylamine dehydrogenase in Methylobacterium extorquens AM1 and Paracoccus denitrificans. However, MauM is required for synthesis of functional methylamine dehydrogenase in ‘M. flagellatum’.
Present address: Institute of Genetics and Selection of Industrial Micoorganisms, 1 Dorozhniy pr. 1, Moscow 114570, Russia.
This article has been cited by other articles:
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  L. Chistoserdova, A. Lapidus, C. Han, L. Goodwin, L. Saunders, T. Brettin, R. Tapia, P. Gilna, S. Lucas, P. M. Richardson, et al. Genome of Methylobacillus flagellatus, Molecular Basis for Obligate Methylotrophy, and Polyphyletic Origin of Methylotrophy J. Bacteriol., June 1, 2007; 189(11): 4020 - 4027. [Abstract] [Full Text] [PDF]
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 A. Y. Chistoserdov Cloning, sequencing and mutagenesis of the genes for aromatic amine dehydrogenase from Alcaligenes faecalis and evolution of amine dehydrogenases Microbiology, August 1, 2001; 147(8): 2195 - 2202. [Abstract] [Full Text] [PDF]
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L. Chistoserdova, L. Gomelsky, J. A. Vorholt, M. Gomelsky, Y. D. Tsygankov, and M. E. Lidstrom Analysis of two formaldehyde oxidation pathways in Methylobacillus flagellatus KT, a ribulose monophosphate cycle methylotroph Microbiology, January 1, 2000; 146(1): 233 - 238. [Abstract] [Full Text]
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