HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by De Groot, P. W. J. Articles by Visser, J. Search for Related Content PubMed PubMed Citation Articles by De Groot, P. W. J. Articles by Visser, J. Agricola Articles by De Groot, P. W. J. Articles by Visser, J.

Isolation of developmentally regulated genes from the edible mushroom Agaricus bisporus

¹ Section Molecular Genetics of Industrial Microorganisms, Wageningen Agricultural University, Dreijenlaan 2, NL-6703 HA, Wageningen, The Netherlands
² Mushroom Experimental Station, PO Box 6042, NL-5960 AA, Horst, The Netherlands

Author for correspondence: Jaap Visser. Tel: +31 317 484439. Fax: +31 317 484011. e-mail: office@algemeen.mgim.wau.nl

ABSTRACT

From a cDNA library, constructed from mushroom primordia, nine cDNAs were isolated which were either induced or specifically expressed during fruit body development and maturation of the basidiomycete Agaricus bisporus. These cDNAs varied in size from 372 to 1019 bp and hybridized to transcripts of 400-1600 nt. Four of the cDNAs were only expressed in the generative phase o: the life cycle while the other five cDNAs were strongly induced but had low steady-state mRNA levels in vegetatively grown mycelium of the hybrid strain Horst U1. An apparent full-length cDNA could be identified by sequence analysis and specified a putative protein homologous to the -subunit of the mitochondrial ATP synthase complex of Saccharomyces cerevisiae and Neurospora crassa. For one of the partial cDNAs, significant homology was found with a family of cell division control proteins, while another partial cDNA appeared to encode a cytochrome P450. All cDNAs, except the presumed cytochrome-P450-specifying cDNA (cypA), hybridized with single copy genes scattered over the Agaricus genome. For the cypA gene, the presence of several additional copies was shown by heterologous hybridizations. Based on changes in expression levels of the fruit-body-induced genes during development coinciding with alterations in morphological appearance of mushrooms, four stages of development were distinguished during growth and maturation of A. bisporus fruit bodies.

This article has been cited by other articles:

				I. Lacourt, S. Duplessis, S. Abba, P. Bonfante, and F. Martin Isolation and Characterization of Differentially Expressed Genes in the Mycelium and Fruit Body of Tuber borchii Appl. Envir. Microbiol., September 1, 2002; 68(9): 4574 - 4582. [Abstract] [Full Text] [PDF]