HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Francois, B. Articles by Courvalin, P. Search for Related Content PubMed PubMed Citation Articles by Francois, B. Articles by Courvalin, P. Agricola Articles by Francois, B. Articles by Courvalin, P.

Conjugative transfer of tet(S) between strains of Enterococcus faecalis is associated with the exchange of large fragments of chromosomal DIMA

Bénédicte Francois

¹Unité des Agents Antibactériens, CNRS EP 10058, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France

*Author for correspondence: Patrice Courvalin. Tel: +33 1 45 68 83 20. Fax: + 33 1 45 68 83 19. e-mail: pcourval@pasteur.fr

ABSTRACT

Summary: The tetracycline resistance determinant tet(S) was first detected in antibiotic multiresistant Listeria monocytogenes BM4210 and subsequently in strains of Enterococcus faecalis. Transfer of tet(S) from clinical isolate E. faecalis BM4242 to E. faecalis strains JH2-2 and OG1RF was found to require the presence in the donor strain of the 55 kb conjugative plasmid pIP825. Comparison of restriction endonuclease generated maps of the donor, the two recipients, and of four transconjugants indicated that transfer of tet(S) (i) was from chromosome to chromosome, (ii) resulted in the acquisition of an approximately 40 kb element in the same chromosomal region and (iii) was associated with the exchange of large chromosomal fragments. Similar observations were made following conjugal transfer of tet(S) from four other E. faecalis clinical isolates.

Present address: Service de lits-porte Pédiatre, Hospital de Bicetre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicetre Cedex, France.

This article has been cited by other articles:

				L. M. Weigel, R. M. Donlan, D. H. Shin, B. Jensen, N. C. Clark, L. K. McDougal, W. Zhu, K. A. Musser, J. Thompson, D. Kohlerschmidt, et al. High-Level Vancomycin-Resistant Staphylococcus aureus Isolates Associated with a Polymicrobial Biofilm Antimicrob. Agents Chemother., January 1, 2007; 51(1): 231 - 238. [Abstract] [Full Text] [PDF]

				A. Villedieu, M. L. Diaz-Torres, N. Hunt, R. McNab, D. A. Spratt, M. Wilson, and P. Mullany Prevalence of Tetracycline Resistance Genes in Oral Bacteria Antimicrob. Agents Chemother., March 1, 2003; 47(3): 878 - 882. [Abstract] [Full Text] [PDF]

				M. POURSHABAN, A. M. FERRINI, V. MANNONI, B. OLIVA, and P. AURELI Transferable tetracycline resistance in Listeria monocytogenes from food in Italy J. Med. Microbiol., July 1, 2002; 51(7): 564 - 597. [Abstract] [Full Text] [PDF]

				L.-K. Ng, M. R. Mulvey, I. Martin, G. A. Peters, and W. Johnson Genetic Characterization of Antimicrobial Resistance in Canadian Isolates of Salmonella Serovar Typhimurium DT104 Antimicrob. Agents Chemother., December 1, 1999; 43(12): 3018 - 3021. [Abstract] [Full Text]

				E. Charpentier and P. Courvalin Antibiotic Resistance in Listeria spp. Antimicrob. Agents Chemother., September 1, 1999; 43(9): 2103 - 2108. [Full Text]