microbiology, Vol 143, 2179-2187, Copyright © 1997 by Society for General Microbiology

ARTICLES

Alanyl-tRNA synthetase gene of the extreme acidophilic chemolithoautotrophic Thiobacillus ferrooxidans is highly homologous to alaS genes from all living kingdoms but cannot be transcribed from its promoter in Escherichia coli

N Guiliani, A Bengrine, F Borne, M Chippaux and V Bonnefoy
Laboratoire de Chimie Bacterienne, Institut de Biologie Structurale et de Microbiologie, Marseille, France.

The alaS gene of Thiobacillus ferrooxidans has been cloned and sequenced and its expression in Escherichia coli and T. ferrooxidans analysed. The same genomic organization to that in E. coli (recA-recX- alaS) has been found in T. ferrooxidans. The recA and alaS genes cannot be transcribed from their own promoters in E. coli. In addition to the well-known homology at the protein level between AlaS proteins from various organisms, a strong homology was found between all the known alaS genes from bacteria, archaea and eucarya. Two regions, one of which corresponds to the catalytic core, are particularly well- conserved at the nucleotide sequence level, a possible indication of strong constraints during evolution on these parts of the genes.

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