HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kang, S. G. Articles by Lee, K. J. Search for Related Content PubMed Articles by Kang, S. G. Articles by Lee, K. J. Agricola Articles by Kang, S. G. Articles by Lee, K. J.

Kinetic analysis of morphological differentiation and protease production in Streptomyces albidoflavus SMF301

Department of Microbiology and Research Centre for Molecular Microbiology, Seoul National University, Seoul 151-742, Korea

¹ Author for correspondence: Kye Joon Lee. Tel: +82 2 880 6705. Fax: +82 2 882 9285/888 4911. e-mail: lkj12345@plaza.snu.ac.kr

ABSTRACT

Summary: The effects of specific growth rate and specific nutrient uptake rate on morphological differentiation of Streptomyces albidoflavus SMF301 were determined in chemostat cultures. Production of three types of proteases: chymotrypsin-like protease (CTP), trypsin-like protease (TLP) and metalloprotease (MTP) were analysed in relation to mycelium growth and spore formation. Production of CTP was closely linked to mycelium growth, whereas spore formation, TLP synthesis and MTP synthesis were inversely related to growth. Evaluation of various kinetic parameters [specific production rates of spores (q_spo), TLP (q_TLP), MTP (q_MTP) and CTP (q_CTP)] showed that mycelium growth rate and CTP production were optimal at 0.1 h^-1, but submerged spore formation, TLP production and MTP production were optimal at 0.025 h^-1. Changes in specific nutrient uptake rates [glucose (q_glu), ammonium ion (q_amn) and phosphate (q_pho)] affected sporulation and protease production; limitation of carbon, nitrogen and phosphate stimulated spore, TLP and MTP production.

This article has been cited by other articles:

				K. E. Wee, C. R. Yonan, and F. N. Chang A new broad-spectrum protease inhibitor from the entomopathogenic bacterium Photorhabdus luminescens Microbiology, December 1, 2000; 146(12): 3141 - 3147. [Abstract] [Full Text] [PDF]