Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Microbiology 143 (1997), 2725-2731; DOI  10.1099/00221287-143-8-2725
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Takeyama, H.
Right arrow Articles by Matsunaga, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Takeyama, H.
Right arrow Articles by Matsunaga, T.
Agricola
Right arrow Articles by Takeyama, H.
Right arrow Articles by Matsunaga, T.

Expression of the eicosapentaenoic acid synthesis gene cluster from Shewanella sp. in a transgenic marine cyanobacterium, Synechococcus sp.

Haruko Takeyama1, Daisuke Takeda1, Kazunaga Yazawa2, Akiko Yamada2 and Tadashi Matsunaga1,1

Tokyo University of Agriculture and Technology, Department of Biotechnology, 2-24-16 Naka-cho, Koganei, Tokyo 184, Japan
Sagami Chemical Research Center, 4-4-1 Nishi-Ohnuma, Sagamihara, Kanagawa 229, Japan

1 Author for correspondence: Tadashi Matsunaga. Tel: +81 423 88 7020. Fax: +81 423 85 7713. e-mail: tmatsuna@cc.taut.ac.jp

ABSTRACT

Summary: The eicosapentaenoic acid (EPA) synthesis gene cluster isolated from a marine bacterium, Shewanella putrefaciens strain SCRC-2738, was cloned and expressed in the marine cyanobacterium Synechococcus sp. A broad-host-range cosmid vector, pJRD215 (10.2 kb, Smr Kmr), was used to clone a 38 kb insert, pEPA, containing the EPA synthesis gene cluster, creating plasmid pJRDEPA (approx. 48 kb). This plasmid was transferred to the cyanobacterial host at a frequency of 2.2 x 10-7. Cyanobacterial transconjugants grown at 29 °C produced 0.12 mg EPA (g dry weight)-1, whereas those grown at 23 °C produced 0.56 mg EPA (g dry weight)-1. The yield was further improved to 0.64 mg (g dry weight)-1 by incubation for 1 d at 17 °C. This is believed to be the first successful cloning and expression of such a large heterologous gene cluster in a marine cyanobacterium.

Keywords: metabolic engineering, marine cyanobacterium, Synechococcus sp., eicosapentaenoic acid (EPA) production, conjugation




This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
H. Okuyama, Y. Orikasa, T. Nishida, K. Watanabe, and N. Morita
Bacterial Genes Responsible for the Biosynthesis of Eicosapentaenoic and Docosahexaenoic Acids and Their Heterologous Expression
Appl. Envir. Microbiol., February 1, 2007; 73(3): 665 - 670.
[Full Text] [PDF]

Home page
 MicrobiologyHome page
E. E. Allen and D. H. Bartlett
Structure and regulation of the omega-3 polyunsaturated fatty acid synthase genes from the deep-sea bacterium Photobacterium profundum strain SS9
Microbiology, June 1, 2002; 148(6): 1903 - 1913.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J.-Q. Liu, T. Dairi, N. Itoh, M. Kataoka, S. Shimizu, and H. Yamada
A Novel Metal-activated Pyridoxal Enzyme with a Unique Primary Structure, Low Specificity D-Threonine Aldolase from Arthrobacter sp. Strain DK-38. MOLECULAR CLONING AND COFACTOR CHARACTERIZATION
J. Biol. Chem., July 3, 1998; 273(27): 16678 - 16685.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1997 Society for General Microbiology.