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Laboratoire de GéAnéAtique Microbienne, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy-en-Josas cedex, France
UnitéA de Biochimie Physiologique, Universite Catholique de Louvain, Place Croix-du-sud 2/20, 1348 Louvain-la-Neuve, Belgium
QIAGEN GmbH, Max-Volmer-Straße 4, 40724 Hilden, Germany
Author for correspondence: Alexe Sorokin. Tel: +33 1 34 65 25 33. Fax: +33 1 34 65 25 21. e-mail: sorokine@biotecjouy.inra.fr
ABSTRACT
Summary: Two regions with sizes 18 900 and 25400 bp, which join previously known contigs containing levRDEFG, aadK and bit genes near 235
of the Bacillus subtilis chromosome, were sequenced. Among others, two genes, which encode proteins homologous to RNA polymerase
-factors, were identified within this region. The gene products designated SigV and SigZ, show the highest homology with
-factors encoded by the gene carQ of Myxococcus xanthus and sigX (formerly orfX20) of B. subtilis, correspondingly. All
-factors which show statistically significant homology to SigV and SigZ, belong to the ECF (extracytoplasmic functions) subfamily. SigV and SigZ do not have N-terminal sequence which prevents such proteins from binding to DNA without RNA polymerase core enzyme.
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