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Microbiology 143 (1997), 2999-3006; DOI  10.1099/00221287-143-9-2999
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Enzymic activity of the mycoparasite Verticillium fungicola on Agaricus bisporus fruit body cell walls

M. Calonje, C. García Mendoza, B. Galán and M. Novaes-Ledieu

Centro de Investigaciones Biológicas, CSIC, Velázquez 144, 28006 Madrid, Spain

Author for correspondence: C. Garcia Mendoza. Tel: +34 1 5611800. Fax: +34 1 5627518.

ABSTRACT

Summary: The in vitro production of different hydrolytic enzyme activities by Verticillium fungicola, a mycoparasite of Agaricus bisporus fruit bodies, was examined in cultures grown with various carbon sources (glucose, fructose, sucrose and A. bisporus cell walls). Several of the identified enzymes were influenced by the carbon source. The results of A. bisporus cell wall digestion by these enzymes in vitro have been compared with V. fungicola infection of A. bisporus fruit bodies in vivo. Evidence supporting mycoparasite enzymic action on the host cell walls is presented.


Keywords: Agaricus bisporus cell walls, lytic enzymes, mycoparasitism, Verticillium fungicola







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Copyright © 1997 Society for General Microbiology.